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http://hdl.handle.net/1942/5333
Title: | Photophysics and fluorimetric titrations of fluorescent ion | Authors: | Boens, N. Cielen, E. VAN WERDE, Kristof de Schryver, F.C. |
Issue Date: | 1999 | Publisher: | PLENUM PUBL CORP | Source: | Journal of fluorescence, 9(2). p. 103-110 | Abstract: | A test based on time-resolved fluorescence experiments (Anal. Biochem.245, 28-37, 1997) allows one to assess the interference of the excited-state association with the fluorimetric determination of the ground-state dissociation constant K-d Of fluorescent ion:indicator complexes. If an inflection point occurs in the plot of the fluorescence signal vs - log[ion] in the ion concentration range where both decay times are invariant, this inflection point can be associated with the correct K-d Here we apply this test to the fluorescent ion indicators SBFO (for Na+), Mag-fura-2 (for Mg2+), and APTRA-BTC (for Ca2+). In all three cases the decay times are invariant in the concentration ranges of the respective ions where the fluorescence titrations show unique inflection points, indicating that the fluorimetrically determined K-d values are the true K-d values. | Keywords: | fluorescence titration; fluorescent ion indicator; decay time analysis; Mag-fura-2; SBFO; APTRA-BTC | Document URI: | http://hdl.handle.net/1942/5333 | ISI #: | 000082923300004 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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