Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/6612
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Raedschelders, G. | - |
dc.contributor.author | Fierens, K. | - |
dc.contributor.author | Sansen, S. | - |
dc.contributor.author | Rombouts, S. | - |
dc.contributor.author | Gebruers, K. | - |
dc.contributor.author | ROBBEN, Johan | - |
dc.contributor.author | Courtin, C.M. | - |
dc.contributor.author | Rabijns, A. | - |
dc.contributor.author | Delcour, J.A. | - |
dc.contributor.author | Van Campenhout, S. | - |
dc.contributor.author | Volckaert, G. | - |
dc.date.accessioned | 2007-12-20T16:09:14Z | - |
dc.date.available | 2007-12-20T16:09:14Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Biochemical and biophysical research communications, 335(2). p. 512-522 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/1942/6612 | - |
dc.description.abstract | Wheat grains contain Triticum aestivum xylanase inhibitor (TAXI) proteins which inhibit microbial xylanases, some of which are used in cereal based food industries. These inhibitors may play a role in plant defence. Among the TAXI isoforms described so far, TAXI-II displays a deviating inhibition specificity pattern. Here, we report on the molecular identity of TAXI-II and the basis of its inhibition specificity. Three candidate TAXI-II encoding sequences were isolated and recombinantly expressed in Pichia pastoris. To identify TAXI-II, the resulting proteins were tested against glycoside hydrolase family (GHF) 11 xylanases of Aspergillus niger (ANX) and Bacillus subtilis (BSX). One of these proteins (rTAXI-IB) inhibited both enzymes, like natural TAXI-I. The other candidates (rTAXI-IIA and rTAXI-IIB) showed an inhibition pattern typical for natural TAXI-II, only clearly inhibiting BSX. Comparative analysis of these highly similar sequences with distinct inhibition activity patterns, combined with information on the structural basis for ANX inhibition by TAXI-I [S. Sansen, C.J. De Ranter, K. Gebruers, K. Brijs, C.M. Courtin, J.A. Delcour, A. Rabijns, Structural basis for inhibition of Aspergillus niger xylanase by Triticum aestivum xylanase inhibitor-I, J. Biol. Chem. 279 (2004) 36022-36028], indicated a crucial role for Pro294 of TAXI-IIA and Gln376 of TAXI-IIB in determining the reduced inhibition activity towards ANX. Consequently.. single point mutants rTAXI-IIA([P294L]) and rTAXI-IIB[Q376H], both displaying the Leu/ His combination corresponding to TAXI-1, were able to inhibit ANX. These results show that TAXI-II inhibition specificity bears on the identity of two key residues at positions 294 and 376, which are involved in the interaction at the -2 glycon subsite and the active site of GHF 11, respectively. (c) 2005 Elsevier Inc. All rights reserved. | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject.other | Triticum aestivum xylanase inhibitor; endo-beta-1,4-xylanase; Taxi gene family; recombinant expression in Pichia pastoris; mutagenesis; inhibitor specificity; enzyme-inhibitor interaction | - |
dc.title | Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 522 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 512 | - |
dc.identifier.volume | 335 | - |
local.bibliographicCitation.jcat | A1 | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.07.103 | - |
dc.identifier.isi | 000231586600037 | - |
item.fulltext | No Fulltext | - |
item.fullcitation | Raedschelders, G.; Fierens, K.; Sansen, S.; Rombouts, S.; Gebruers, K.; ROBBEN, Johan; Courtin, C.M.; Rabijns, A.; Delcour, J.A.; Van Campenhout, S. & Volckaert, G. (2005) Molecular identification of wheat endoxylanase inhibitor TAXI-II and the determinants of its inhibition specificity. In: Biochemical and biophysical research communications, 335(2). p. 512-522. | - |
item.contributor | Raedschelders, G. | - |
item.contributor | Fierens, K. | - |
item.contributor | Sansen, S. | - |
item.contributor | Rombouts, S. | - |
item.contributor | Gebruers, K. | - |
item.contributor | ROBBEN, Johan | - |
item.contributor | Courtin, C.M. | - |
item.contributor | Rabijns, A. | - |
item.contributor | Delcour, J.A. | - |
item.contributor | Van Campenhout, S. | - |
item.contributor | Volckaert, G. | - |
item.accessRights | Closed Access | - |
crisitem.journal.issn | 0006-291X | - |
crisitem.journal.eissn | 1090-2104 | - |
Appears in Collections: | Research publications |
SCOPUSTM
Citations
20
checked on Sep 7, 2020
WEB OF SCIENCETM
Citations
22
checked on May 16, 2024
Page view(s)
60
checked on Jun 28, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.