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Title: | Dehydroascorbate uptake is impaired in the early response of Arabidopsis plant cell cultures to cadmium | Authors: | HOREMANS, Nele Raeymaekers, Tine Van Beek, Kim Nowocin, Anna Blust, Ronny Broos, Katleen CUYPERS, Ann VANGRONSVELD, Jaco Guisez, Yves |
Issue Date: | 2007 | Publisher: | OXFORD UNIV PRESS | Source: | JOURNAL OF EXPERIMENTAL BOTANY, 58(15-16). p. 4307-4317 | Abstract: | The balance between antioxidants, such as ascorbate (ASC) and glutathione, and oxidative reactive oxygen species (ROS) is known to play a pivotal role in the response of plant cells to abiotic stress. Here cell cultures of Arabidopsis thaliana were investigated with regard to their response to elevated levels of cadmium. At concentrations <100 mu M, Cd induces a rapid and concentration-dependent H2O2 accumulation. This response could be inhibited by diphenylene iodonium (DPI, 20 mu M). Reverse transcription-PCR analysis of three RBOH (respiratory burst oxidase homologues) genes showed an increased transcription of RBOHF after 15 min. No change in ASC concentration was observed during the first 3 h after Cd addition. In contrast, glutathione levels completely diminished within 1 h. This drop could be attributed to an increase in phytochelatin 4. At the plasma membrane, Cd further induced a significant decrease in dehydroascorbate (DHA) uptake activity (up to 90% inhibition after 4 h). This decrease is not present when cells are treated with LaCl3 before exposure to CdCl2. LaCl3 is a typical inhibitor of Ca channels and prevents Cd uptake in these cells as well as the Cd-induced ROS production. Therefore, these results appear to indicate that Cd uptake is a prerequisite for the change in DHA transport activity. However, DPI did not prevent the drop in DHA uptake activity present in Cd-treated Arabidopsis cells, indicating that this response seems to be independent of the Cd-induced H2O2 production. | Notes: | Univ Antwerp, Dept Biol Plant Physiol, B-2020 Antwerp, Belgium. Univ Antwerp, Dept Biol Ecophysiol Biochem & Toxicol, B-2020 Antwerp, Belgium. Hasselt Univ, Ctr Environm Sci Environm Biol, B-3590 Diepenbeek, Belgium.Horemans, N, Univ Antwerp, Dept Biol Plant Physiol, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.nele.horemans@ua.ac.be | Keywords: | Arabidopsis thaliana; cell cultures; ascorbate; cadmium; DHA uptake; glutathione; reactive oxygen species;Arabidopsis thaliana cell cultures; ascorbate cadmium; DHA uptake; glutathione; reactive oxygen species | Document URI: | http://hdl.handle.net/1942/8001 | ISSN: | 0022-0957 | e-ISSN: | 1460-2431 | DOI: | 10.1093/jxb/erm291 | ISI #: | 000253095900026 | Rights: | (C) 2007 The Author(s). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
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