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http://hdl.handle.net/1942/8008
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DC Field | Value | Language |
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dc.contributor.author | JANS, Frank | - |
dc.contributor.author | AMELOOT, Marcel | - |
dc.contributor.author | WOUTERS, P. | - |
dc.contributor.author | STEELS, Paul | - |
dc.date.accessioned | 2008-03-17T10:10:49Z | - |
dc.date.available | 2008-03-17T10:10:49Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 86(1-2). p. 36-45 | - |
dc.identifier.issn | 0008-4212 | - |
dc.identifier.uri | http://hdl.handle.net/1942/8008 | - |
dc.description.abstract | The cellular pathophysiology of renal ischemia-reperfusion injury was investigated in primary cell cultures from rabbit medullary thick ascending limb (MTAL). Metabolic inhibition (MI) was achieved with cyanide and 2-deoxy-glucose. Sixty minutes of MI caused a profound but reversible decrease in intracellular concentration of ATP ([ATP](i)). Intracellular pH (pH(i)) first decreased after initiation of MI, followed by a transient alkalinization. When [ATP](i) reached its lowest value (<1% of control), the cells slowly acidified to reach a stable pH(i) of 6.92 after 50 min of MI. In the presence of EIPA (10 mu mol/L), the pattern of changes in pH(i) was unchanged and acidification was not increased, indicating that the Na+/H+ exchangers were inactive during ATP depletion. When inorganic phosphate (P-i) or Na+ was omitted from the apical solutions during MI, the transient alkalinization was no longer observed and the cytosol slowly acidified. Experiments on Na+-dependent alkalinizations revealed the presence of a Na-P-i cotransporter in the apical cell membrane. With indirect immunofluorescence, the Na-P-i cotransporter expressed in these primary cell cultures could be identified as Na-P-i type I. Although the exact physiological role of Na-P-i type I still is unresolved, these experiments demonstrate that apical Na-P-i type I activity is increased at the onset of ATP depletion in MTAL cells. | - |
dc.language.iso | en | - |
dc.publisher | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS | - |
dc.subject.other | MTAL; cell culture; ischemia; metabolic inhibition; intracellular pH; alkalinization; Na-P-i cotransporter | - |
dc.title | Na-P-i cotransporter type I activity causes a transient intracellular alkalinization during ATP depletion in rabbit medullary thick ascending limb cells | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 45 | - |
dc.identifier.issue | 1-2 | - |
dc.identifier.spage | 36 | - |
dc.identifier.volume | 86 | - |
local.format.pages | 10 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Catholic Univ Louvain, Dept Anaesthesiol, Univ Hosp Gasthuisberg, B-3000 Louvain, Belgium.Jans, F, Univ Hasselt, Dept Physiol, Biomed Res Inst, B-3590 Diepenbeek, Belgium.frank.jans@hotmaii.com | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1139/Y07-114 | - |
dc.identifier.isi | 000253544900006 | - |
item.fulltext | No Fulltext | - |
item.contributor | JANS, Frank | - |
item.contributor | AMELOOT, Marcel | - |
item.contributor | WOUTERS, P. | - |
item.contributor | STEELS, Paul | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2009 | - |
item.fullcitation | JANS, Frank; AMELOOT, Marcel; WOUTERS, P. & STEELS, Paul (2008) Na-P-i cotransporter type I activity causes a transient intracellular alkalinization during ATP depletion in rabbit medullary thick ascending limb cells. In: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 86(1-2). p. 36-45. | - |
crisitem.journal.issn | 0008-4212 | - |
crisitem.journal.eissn | 1205-7541 | - |
Appears in Collections: | Research publications |
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