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http://hdl.handle.net/1942/8298
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DC Field | Value | Language |
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dc.contributor.author | LEMMENS, Evi | - |
dc.contributor.author | Schijns, OEMG | - |
dc.contributor.author | BEULS, Emile | - |
dc.contributor.author | HOOGLAND, Govert | - |
dc.date.accessioned | 2008-05-27T10:26:11Z | - |
dc.date.available | 2008-05-27T10:26:11Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | EPILEPSIA, 49(5). p. 853-860 | - |
dc.identifier.issn | 0013-9580 | - |
dc.identifier.uri | http://hdl.handle.net/1942/8298 | - |
dc.description.abstract | Purpose: Febrile seizures (FS) are early-life seizures thought to play a role in epileptogenesis. By labeling cells that were dividing immediately following experimental FS, we previously demonstrated that significantly more of these newborn cells in the dentate gyrus (DG) survived 8 weeks later, relative to animals that did not experience FS. The purpose of the present study was to determine the long-term fate of these newborn cells. Methods: On postnatal day (PN) 10, hyperthermia-induced seizures (HT, +/- 42 degrees C core temperature) were evoked in Sprague-Dawley rats and littermates were used as normothermia controls (NT, +/- 35 degrees C core temperature). From PN11 to PN16, rats were injected with bromodeoxyuridine (BrdU) to label dividing cells. At PN66, we evaluated the number of BrdU-labeled cells in the DG that colocalized with the neuronal marker NeuN, glial marker glial fibrillary acidic protein (GFAP), neuronal excitatory amino acid transporter 3 (EAAT3), GABAergic neuronal marker glutamic acid decarboxylase 67 (GAD67) or microglia marker tomato lectin (TL). Results: In all rats, almost all BrdU-labeled cells in the DG, that showed double-labeling, colocalized with NeuN, and rarely with GFAP, GAD67, or TL. In NT controls and HT rats that did not experience seizures ("HT-no seizures"), similar to 23% of BrdU-labeled cells colocalized with EAAT3, which was significantly different from 14% in HT rats that did experience seizures (HT + FS). Discussion: Early-life seizures decrease the population of newborn cells that survive and mature into EAAT3-positive neurons and do not affect the GABAergic cell population. This may affect hippocampal physiology in young adulthood. | - |
dc.language.iso | en | - |
dc.publisher | BLACKWELL PUBLISHING | - |
dc.subject.other | febrile seizures; hyperthermia; hippocampus; neurogenesis; glutamate transporter | - |
dc.title | Cytogenesis in the dentate gyrus after neonatal hyperthermia-induced seizures: What becomes of surviving cells? | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 860 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 853 | - |
dc.identifier.volume | 49 | - |
local.format.pages | 8 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Maastricht Univ, Div Neurosci, Dept Psychiat & Neuropsychol, NL-6200 MD Maastricht, Netherlands. Univ Hosp Maastricht, Dept Neurosurg, Maastricht, Netherlands. European Grad Sch Neurosci EURON Maastricht, Maastricht, Netherlands. Hasselt Univ, Dept Anat & Mat Res, Diepenbeek, Belgium. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1111/j.1528-1167.2007.01476.x | - |
dc.identifier.isi | 000255480500013 | - |
item.fulltext | No Fulltext | - |
item.contributor | LEMMENS, Evi | - |
item.contributor | Schijns, OEMG | - |
item.contributor | BEULS, Emile | - |
item.contributor | HOOGLAND, Govert | - |
item.fullcitation | LEMMENS, Evi; Schijns, OEMG; BEULS, Emile & HOOGLAND, Govert (2008) Cytogenesis in the dentate gyrus after neonatal hyperthermia-induced seizures: What becomes of surviving cells?. In: EPILEPSIA, 49(5). p. 853-860. | - |
item.accessRights | Closed Access | - |
item.validation | ecoom 2009 | - |
crisitem.journal.issn | 0013-9580 | - |
crisitem.journal.eissn | 1528-1167 | - |
Appears in Collections: | Research publications |
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