Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/8565
Title: | Rapid, postmortem 9.4 T MRI of spinal cord injury: Correlation with histology and survival times | Authors: | Scholtes, Felix Phan-Ba, Remy THEUNISSEN, Evi ADRIAENSENS, Peter Brook, Gary Franzen, Rachelle Bouhy, Delphine GELAN, Jan Martin, Didier Schoenen, Jean |
Issue Date: | 2008 | Publisher: | ELSEVIER SCIENCE BV | Source: | JOURNAL OF NEUROSCIENCE METHODS, 174(2). p. 157-167 | Abstract: | High field magnetic resonance imaging (MRI) has been increasingly used to assess experimental spinal cord injury (SCI). In the present investigation, after partial spinal cord injury and excision of the whole spine, pathological changes of the spinal cord were studied in spinal cord-spine blocks, from the acute to the chronic state (24 h to 5 months). Using proton density (PD) weighted imaging parameters at a magnetic field strength of 9.4 tesla (T), acquisition times ranging from < 1 to 10 h per specimen were used. High in-plane pixel resolution (68 and 38 mu m, respectively) was obtained, as well as high signal-to-noise ratio (SNR), which is important for optimal contrast settings. The quality of the resulting MR images was demonstrated by comparison with histology. The cord and the lesion were shown in their anatomical Surroundings, detecting cord swelling in the acute phase (24 h to 1 week) and cord atrophy at the chronic stage. Haemorrhage was detected as hypo-intense signal. Oedema, necrosis and scarring were hyper-intense but could not be distinguished. Histology confirmed that the anatomical delimitation of the lesion extent by MRI was precise, both with high and moderate resolution. The present investigation thus demonstrates the precision of spinal cord MRI at different survival delays after compressive Partial SCI and establishes efficient imaging parameters for postmortem PD MRI. (C) 2008 Elsevier B.V. All rights reserved. | Notes: | [Scholtes, Felix; Phan-Ba, Remy; Franzen, Rachelle; Bouhy, Delphine; Schoenen, Jean] Univ Liege, Ctr Cellular & Mol Neurobiol CNCM, Dept Neuroanat, B-4000 Liege, Belgium. [Scholtes, Felix; Martin, Didier] CHU, Dept Neurosurg, B-4000 Liege, Belgium. [Adriaensens, Peter; Gelan, Jan] Univ Hasselt, Inst Mat Res IMO, Div Chem, B-3590 Diepenbeek, Belgium. [Theunissen, Evi] Univ Hasselt, Biomed Res Inst BIOMED, B-3590 Diepenbeek, Belgium. [Brook, Gary] Rhein Westfal TH Aachen, Dept Neuropathol, D-52057 Aachen, Germany. [Schoenen, Jean] Citadelle Hosp, Univ Dept Neurol, B-4000 Liege, Belgium. | Keywords: | Histology; Magnetic resonance imaging; Postmortem; Rat; Spinal cord injury | Document URI: | http://hdl.handle.net/1942/8565 | ISSN: | 0165-0270 | e-ISSN: | 1872-678X | DOI: | 10.1016/j.jneumeth.2008.06.038 | ISI #: | 000259905600001 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2009 |
Appears in Collections: | Research publications |
Show full item record
SCOPUSTM
Citations
3
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
7
checked on Oct 14, 2024
Page view(s)
80
checked on Jun 28, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.