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http://hdl.handle.net/1942/8996
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DC Field | Value | Language |
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dc.contributor.author | VANHOUDT, Nathalie | - |
dc.contributor.author | VANDENHOVE, Hildegarde | - |
dc.contributor.author | SMEETS, Karen | - |
dc.contributor.author | REMANS, Tony | - |
dc.contributor.author | Van Hees, May | - |
dc.contributor.author | Wannijn, Jean | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.date.accessioned | 2008-12-09T09:58:57Z | - |
dc.date.available | 2008-12-09T09:58:57Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46(11). p. 987-996 | - |
dc.identifier.issn | 0981-9428 | - |
dc.identifier.uri | http://hdl.handle.net/1942/8996 | - |
dc.description.abstract | The production of reactive oxygen species (ROS) and the induction of the antioxidative defense mechanism are very important in heavy metal toxicity. In this study, biological effects induced after uranium contamination were investigated for Arabidopsis thaliana. Three-week-old seedlings were exposed for 4 days to 100 mu M U in an adjusted Hoagland solution. Uranium exposure caused a decreased growth of leaves (38%) and roots (70%) and a modified nutrient profile was observed. Investigation of lipid peroxidation products indicated a significant increase of membrane damage. Important ROS-producing and -scavenging enzymes were studied at transcriptional and protein level to investigate the importance of the ROS-signature in uranium toxicity. Elevated gene expression was observed for NADPH-oxidase, a ROS-producing enzyme. Changes in gene expression for different ROS-scavenging enzymes as Cu/ZnSOD. FeSOD and APX were also observed. Analysis of enzyme capacities showed little effects after uranium contamination. Higher ascorbate levels in uranium exposed leaves suggested an increase of antioxidative defense via the ascorbate-glutathione pathway after uranium exposure. Theoretical calculations indicated rapid formation of uranium-phosphate precipitates if normal phosphate concentrations are used. Precipitation tests recommend the use of 25 mu M P in combination with 100 mu M U to inhibit uranium precipitation. Because this combination was used for uranium toxicity investigation, the influence of this low phosphate concentration on plant growth and oxidative stress had to be evaluated. Minor differences between low phosphate (25 mu M P) and high phosphate ( 100 mu M P) treatments were observed justifying the use of the low phosphate concentration in combination with uranium. (C) 2008 Elsevier Masson SAS. All rights reserved. | - |
dc.description.sponsorship | This work was supported by a PhD grant for Nathalie Vanhoudt from the Belgian Nuclear Research Centre (SCKCEN). | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | - |
dc.rights | (C) 2008 Elsevier Masson SAS. All rights reserved. | - |
dc.subject.other | Arabidopsis thaliana; Enzyme capacity; Gene expression; Oxidative stress; Phosphate fertilization; Uranium toxicity | - |
dc.subject.other | arabidopsis thaliana; enzyme capacity; gene expression; oxidative stress; phosphate fertilization; uranium toxicity | - |
dc.title | Effects of uranium and phosphate concentrations on oxidative stress related responses induced in Arabidopsis thaliana | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 996 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 987 | - |
dc.identifier.volume | 46 | - |
local.format.pages | 10 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Vanhoudt, Nathalie; Vandenhove, Hildegarde; Van Hees, May; Wannijn, Jean] CEN SCK, Biosphere Impact Studies, Belgian Nucl Res Ctr, B-2400 Mol, Belgium. [Vanhoudt, Nathalie; Smeets, Karen; Remans, Tony; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, B-3590 Diepenbeek, Belgium. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.plaphy.2008.06.003 | - |
dc.identifier.isi | 000260809800007 | - |
item.validation | ecoom 2009 | - |
item.contributor | VANHOUDT, Nathalie | - |
item.contributor | VANDENHOVE, Hildegarde | - |
item.contributor | SMEETS, Karen | - |
item.contributor | REMANS, Tony | - |
item.contributor | Van Hees, May | - |
item.contributor | Wannijn, Jean | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.contributor | CUYPERS, Ann | - |
item.accessRights | Restricted Access | - |
item.fullcitation | VANHOUDT, Nathalie; VANDENHOVE, Hildegarde; SMEETS, Karen; REMANS, Tony; Van Hees, May; Wannijn, Jean; VANGRONSVELD, Jaco & CUYPERS, Ann (2008) Effects of uranium and phosphate concentrations on oxidative stress related responses induced in Arabidopsis thaliana. In: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46(11). p. 987-996. | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 0981-9428 | - |
crisitem.journal.eissn | 1873-2690 | - |
Appears in Collections: | Research publications |
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Vangronsveld.pdf Restricted Access | Published version | 402.47 kB | Adobe PDF | View/Open Request a copy |
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