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http://hdl.handle.net/1942/9284
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DC Field | Value | Language |
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dc.contributor.author | Lindemans, Marleen | - |
dc.contributor.author | LIU, Feng | - |
dc.contributor.author | Janssen, Tom | - |
dc.contributor.author | Husson, Steven J. | - |
dc.contributor.author | Mertens, Inge | - |
dc.contributor.author | Gaede, Gerd | - |
dc.contributor.author | Schoofs, Liliane | - |
dc.date.accessioned | 2009-03-02T09:49:54Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(5). p. 1642-1647 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/1942/9284 | - |
dc.description.abstract | In mammals, hypothalamic gonadotropin-releasing hormone (GnRH) is a neuropeptide that stimulates the release of gonadotropins from the anterior pituitary. The existence of a putative functional equivalent of this reproduction axis in protostomian invertebrates has been a matter of debate. In this study, the ligand for the GnRH receptor in the nematode Caenorhabditis elegans (Ce-GnRHR) was found using a bioinformatics approach. The peptide and its precursor are reminiscent of both insect adipokinetic hormones and GnRH-preprohormone precursors from tunicates and higher vertebrates. We cloned the AKH-GnRH-like preprohormone and the Ce-GnRHR and expressed the GPCR in HEK293T cells. The GnRHR was activated by the C. elegans AKH-GnRH-like peptide (EC50 = 150 nM) and by Drosophila AKH and other nematode AKH-GnRHs that we found in EST databases. Analogous to both insect AKH receptor and vertebrate GnRH receptor signaling, Ce-AKH-GnRH activated its receptor through a G alpha(q) protein with Ca2+ as a second messenger. Gene silencing of Ce-GnRHR, Ce-AKH-GnRH, or both resulted in a delay in the egg-laying process, comparable to a delay in puberty in mammals lacking a normal dose of GnRH peptide or with a mutated GnRH precursor or receptor gene. The present data support the view that the AKH-GnRH signaling system probably arose very early in metazoan evolution and that its role in reproduction might have been developed before the divergence of protostomians and deuterostomians. | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject.other | C. elegans; G protein-coupled receptor; molecular evolution; neuropeptide; neuroendocrinology | - |
dc.title | Adipokinetic hormone signaling through the gonadotropin-releasing hormone receptor modulates egg-laying in Caenorhabditis elegans | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1647 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1642 | - |
dc.identifier.volume | 106 | - |
local.format.pages | 6 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | [Lindemans, Marleen; Janssen, Tom; Husson, Steven J.; Mertens, Inge; Schoofs, Liliane] Katholieke Univ Leuven, Funct Genom & Prote Res Unit, B-3000 Louvain, Belgium. [Liu, Feng] Hasselt Univ, Transportat Res Inst, Data Anal & Modeling Grp, B-3590 Diepenbeek, Belgium. [Gaede, Gerd] Univ Cape Town, Dept Zool, ZA-7701 Rondebosch, South Africa. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1073/pnas.0809881106 | - |
dc.identifier.isi | 000263074600063 | - |
item.fulltext | No Fulltext | - |
item.accessRights | Closed Access | - |
item.fullcitation | Lindemans, Marleen; LIU, Feng; Janssen, Tom; Husson, Steven J.; Mertens, Inge; Gaede, Gerd & Schoofs, Liliane (2009) Adipokinetic hormone signaling through the gonadotropin-releasing hormone receptor modulates egg-laying in Caenorhabditis elegans. In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(5). p. 1642-1647. | - |
item.validation | ecoom 2010 | - |
item.contributor | Lindemans, Marleen | - |
item.contributor | LIU, Feng | - |
item.contributor | Janssen, Tom | - |
item.contributor | Husson, Steven J. | - |
item.contributor | Mertens, Inge | - |
item.contributor | Gaede, Gerd | - |
item.contributor | Schoofs, Liliane | - |
crisitem.journal.issn | 0027-8424 | - |
crisitem.journal.eissn | 1091-6490 | - |
Appears in Collections: | Research publications |
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