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Title: Autocrine/paracrine activation of the GABA(A) receptor inhibits the proliferation of neurogenic polysialylated neural cell adhesion molecule-positive (PSA-NCAM(+)) precursor cells from postnatal striatum
Authors: Nguyen, L
Malgrange, B.
Breuskin, I
Bettendorff, L
Moonen, G
Belachew, S.
RIGO, Jean-Michel 
Issue Date: 2003
Source: JOURNAL OF NEUROSCIENCE, 23(8). p. 3278-3294
Abstract: GABA and its type A receptor (GABA(A)R) are present in the immature CNS and may function as growth-regulatory signals during the development of embryonic neural precursor cells. In the present study, on the basis of their isopycnic properties in a buoyant density gradient, we developed an isolation procedure that allowed us to purify proliferative neural precursor cells from early postnatal rat striatum, which expressed the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). These postnatal striatal PSA-NCAM(+) cells were shown to proliferate in the presence of epidermal growth factor (EGF) and formed spheres that preferentially generated neurons in vitro. We demonstrated that PSA-NCAM(+) neuronal precursors from postnatal striatum expressed GABA(A)R subunits in vitro and in situ. GABA elicited chloride currents in PSA-NCAM(+) cells by activation of functional GABA(A)R that displayed a typical pharmacological profile. GABA(A)R activation in PSA-NCAM(+) cells triggered a complex intracellular signaling combining a tonic inhibition of the mitogen-activated protein kinase cascade and an increase of intracellular calcium concentration by opening of voltage-gated calcium channels. We observed that the activation of GABA(A)R in PSA-NCAM(+) neuronal precursors from postnatal striatum inhibited cell cycle progression both in neurospheres and in organotypic slices. Furthermore, postnatal PSA-NCAM(+) striatal cells synthesized and released GABA, thus creating an autocrine/paracrine mechanism that controls their proliferation. We showed that EGF modulated this autocrine/paracrine loop by decreasing GABA production in PSA-NCAM(+) cells. This demonstration of GABA synthesis and GABA(A)R function in striatal PSA-NCAM(+) cells may shed new light on the understanding of key extrinsic cues that regulate the developmental potential of postnatal neuronal precursors in the CNS.
Notes: Univ Liege, Ctr Cellular & Mol Neurobiol, B-4020 Liege, Belgium. Univ Liege, CHU Sart Tilman, Dept Neurol, B-4000 Liege, Belgium.
Keywords: GABA(A) receptors; newborn rat striata; proliferation; PSA-NCAM; whole-cell patch-clamp; RT-PCR; HPLC; immunocytochemistry
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ISSN: 0270-6474
e-ISSN: 1529-2401
ISI #: 000182475200022
Category: A1
Type: Journal Contribution
Appears in Collections:Research publications

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