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Title: | Identification and comparative analysis of the structural proteomes of phi KZ and EL, two giant Pseudomonas aeruginosa bacteriophages | Authors: | Lecoutere, Elke Ceyssens, Pieter-Jan Miroshnikov, Konstantin A. Mesyanzhinov, Vadim V. Krylov, Victor N. NOBEN, Jean-Paul ROBBEN, Johan Hertveldt, Kirsten Volckaert, Guido Lavigne, Rob |
Issue Date: | 2009 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | PROTEOMICS, 9(11). p. 3215-3219 | Abstract: | Giant bacteriophages phi KZ and EL of Pseudomonas aeruginosa contain 62 and 64 structural proteins, respectively, identified by ESI-MS/MS on total virion particle proteins. These identifications verify gene predictions and delineate the genomic regions dedicated to phage assembly and capsid formation (30 proteins were identified from a tailless phi KZ mutant). These data form the basis for future structural studies and provide insights into the relatedness of these large phages. The 4 KZ structural proteome strongly correlates to that of Pseudomonas chlororaphis bacteriophage 201 phi 2-1. Phage EL is more distantly related, shown by its 26 non-conserved structural proteins and the presence of genomic inversions. | Notes: | [Lecoutere, Elke; Ceyssens, Pieter-Jan; Hertveldt, Kirsten; Volckaert, Guido; Lavigne, Rob] Katholieke Univ Leuven, Div Gene Technol, B-3001 Louvain, Belgium. [Miroshnikov, Konstantin A.; Mesyanzhinov, Vadim V.] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia. [Krylov, Victor N.] State Inst Genet & Select Ind Microorganisms, Moscow, Russia. [Noben, Jean-Paul; Robben, Johan] Univ Hasselt, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul; Robben, Johan] Univ Hasselt, Sch Life Sci, Transnat Univ Limburg, Diepenbeek, Belgium. | Keywords: | Bacteriophages; ESI-MS/MS; Structural proteome | Document URI: | http://hdl.handle.net/1942/9795 | ISSN: | 1615-9853 | e-ISSN: | 1615-9861 | DOI: | 10.1002/pmic.200800727 | ISI #: | 000267414500025 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2010 |
Appears in Collections: | Research publications |
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