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Title: | Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase | Authors: | Lebeer, Sarah Verhoeven, Tine L. A. Francius, Gregory Schoofs, Geert LAMBRICHTS, Ivo Dufrene, Yves Vanderleyden, Jos De Keersmaecker, Sigrid C. J. |
Issue Date: | 2009 | Publisher: | AMER SOC MICROBIOLOGY | Source: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(11). p. 3554-3563 | Abstract: | Cell surface polysaccharides have an established role as virulence factors in human bacterial pathogens. Less documented are the biosynthesis and biological functions of surface polysaccharides in beneficial bacteria. We identified a gene cluster that encodes the enzymes and regulatory and transporter proteins for the different steps in the biosynthesis of extracellular polysaccharides (EPS) of the well-documented probiotic strain Lactobacillus rhamnosus GG. Subsequent mutation of the welE gene, encoding the priming glycosyltransferase within this cluster, and comparative phenotypic analyses of wild-type versus mutant strains confirmed the specific function of this gene cluster in the biosynthesis of high-molecular-weight, galactose-rich heteropolymeric EPS molecules. The phenotypic analyses included monomer composition determination, estimation of the polymer length of the isolated EPS molecules, and single-molecule force spectroscopy of the surface polysaccharides. Further characterization of the welE mutant also showed that deprivation of these long, galactose-rich EPS molecules results in an increased adherence and biofilm formation capacity of L. rhamnosus GG, possibly because of less shielding of adhesins such as fimbria-like structures. | Notes: | [Lebeer, Sarah; Verhoeven, Tine L. A.; Schoofs, Geert; Vanderleyden, Jos; De Keersmaecker, Sigrid C. J.] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium. [Francius, Gregory; Dufrene, Yves] Univ Catholique Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium. [Lambrichts, Ivo] Univ Hasselt, Histol Lab, Biomed Res Inst, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/9811 | ISSN: | 0099-2240 | e-ISSN: | 1098-5336 | DOI: | 10.1128/AEM.02919-08 | ISI #: | 000266345800021 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2010 |
Appears in Collections: | Research publications |
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