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Title: Concept for a solid-state multi-parameter sensor system for cell-culture monitoring
Authors: Baecker, M.
Beging, S.
Biselli, M.
Poghossian, A.
Wang, J.
Zang, W.
WAGNER, Patrick 
Schoening, M. J.
Issue Date: 2009
Source: ELECTROCHIMICA ACTA, 54(25). p. 6107-6112
Abstract: In this study. a concept for a silicon-based modular solid-state sensor system for inline multi-parameter monitoring of cell-culture fermentation processes is presented. The envisaged multi-parameter sensor system consists of two identical sensor modules and is intended for continuous quantification of up to five (bio-)chemical and physical parameters, namely, glucose and glutamine concentration, pH value, electrolyte conductivity and temperature by applying different transducer principles and/or different operation modes. Experimental results for the field-effect electrolyte-insulator-semiconductor (EIS) sterilisable pH sensor and electrolyte conductivity sensor based on interdigitated electrodes are presented. The ongoing autoclaving does not have any significant impact on the pH-sensitive properties of a Ta(2)O(5-)gate EIS sensor. Even after 30 autoclaving cycles, the pH sensors show a clear pH response and nearly linear calibration curve with a slope of 57 +/- 1 mV/pH. Additional scanning electron microscopy and ellipsometric investigations do not show any visible surface degradation or changes in the thickness of the pH-sensitive Ta2O5 layer. The preliminary results demonstrate the suitability of the developed EIS, sensor for an inline pH measurement during a fermentation process. in addition, interdigitated electrodes of different geometries serving as electrolyte conductivity sensor have been tested for measurements in relatively high ionic-strength solutions. (C) 2009 Elsevier Ltd. All rights reserved.
Notes: [Baecker, M.; Beging, S.; Biselli, M.; Poghossian, A.; Wang, J.; Schoening, M. J.] Aachen Univ Appl Sci, INB, Aachen, Germany. [Baecker, M.; Beging, S.; Poghossian, A.; Wang, J.; Schoening, M. J.] Res Ctr Julich GmbH, Inst Bio & Nanosyst IBN 2, Julich, Germany. [Zang, W.] HiTec Zang GmbH, Herzogenrath, Germany. [Wagner, P.] Hasselt Univ, Inst Mat Res, Diepenbeek, Belgium.
Keywords: Fermentation process; pH; Field-effect; Electrolyte conductivity; Multi-parameter detection
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ISSN: 0013-4686
e-ISSN: 1873-3859
DOI: 10.1016/j.electacta.2009.02.091
ISI #: 000270347200026
Category: A1
Type: Journal Contribution
Validations: ecoom 2010
Appears in Collections:Research publications

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