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http://hdl.handle.net/1942/1002
Title: | Profiling the autoantibody repertoire by serological antigen selection | Authors: | SOMERS, Veerle GOVARTS, Cindy HELLINGS, Niels Hupperts, R. STINISSEN, Piet |
Issue Date: | 2005 | Publisher: | Elsevier | Source: | JOURNAL OF AUTOIMMUNITY, 25(3). p. 223-228 | Abstract: | The identification of disease related autoantigens targeted by pathogenic T- and B-cell responses is crucial for the development of improved therapies for autoimmune diseases. To identify immunogenic targets recognized by the humoral immune response, we have recently applied a novel and powerful molecular approach, named ‘serological antigen selection’ (SAS). This method involves the display of a cDNA expression library on filamentous phage and subsequent selection on patient immunoglobulin G (IgG). In the present study, we have cloned a cDNA repertoire from a multiple sclerosis (MS) patient in pVI phage display vectors and performed selections on pooled MS cerebrospinal fluid (CSF) samples immobilized with anti-human IgG. To further streamline this procedure, we report an optimized SAS procedure in which we have successfully established methods for enrichment of MS-specific candidate antigens. In conclusion, the broad applicability of the SAS method makes it a highly promising method for investigating the autoimmune repertoire. | Keywords: | Serological antigen selection; Filamentous phage; cDNA display; Multiple sclerosis; Autoantibody repertoire; Autoimmune diseases | Document URI: | http://hdl.handle.net/1942/1002 | ISSN: | 0896-8411 | e-ISSN: | 1095-9157 | DOI: | 10.1016/j.jaut.2005.09.023 | ISI #: | 000233891200008 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2006 |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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Profiling.pdf | Peer-reviewed author version | 309.81 kB | Adobe PDF | View/Open |
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