Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/10164
Title: | Glycine enhances microglial calcium fluxes: A possible role for neutral amino acid transporters | Authors: | NOTELAERS, Kristof | Advisors: | RIGO, J. | Issue Date: | 2009 | Publisher: | tUL Diepenbeek | Abstract: | Based on recent electrophysiological data, we hypothesized a possible role for neutral amino acid transporters in glycine modulation of microglial activity. Since it has been demonstrated that calcium (Ca2+) signals correlate to microglial activity, Ca2+ signal modulation was investigated. Results showed that glycine enhanced microglial Ca2+ signals induced by ATP, LPS and thapsigargin. These effects were GlyR-independent and could be blocked by the competitive amino acid transporter substrate AIB ('-amino-isobutyric acid). Glycine immunostaining also showed an AIB-sensitive uptake of glycine in microglial cells. Together, these results provide strong arguments for neutral amino acid transporter involvement in glycine enhancement of microglial Ca2+ signals. Additional results obtained using ELISA assays, showed enhanced TNF-' production in glycine conditions. This further confirms that the immune function of microglial cells is enhanced by glycine and that the glycine effects on microg | Notes: | 2de masterjaar in de biomedische wetenschappen - klinische moleculaire wetenschappen | Document URI: | http://hdl.handle.net/1942/10164 | Category: | T2 | Type: | Theses and Dissertations |
Appears in Collections: | Master theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
04217172008249c.pdf | 1.11 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.