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Title: Analysis of Mitochondrial pH and Ion Concentrations
Authors: VAN DE VEN, Martin 
BALUT, Corina 
BARON, Szilvia 
SMETS, Ilse 
AMELOOT, Marcel 
Issue Date: 2010
Publisher: Humana Press
Source: Papovski, DB (Ed.) Life Cell Imaging, p. 275-309.
Series/Report: Methods in Molecular Biology
Series/Report no.: 591
Abstract: Detailed practical information is provided with emphasis on mapping cytosolic and mitochondrial pH, mitochondrial Na+, and briefly also aspects related to mitochondrial Ca2+ measurements in living cells, as grown on (un)coated glass coverslips. This chapter lists (laser scanning confocal) microscope instrumentation and setup requirements for proper imaging conditions, cell holders, and an easy-to-use incubator stage. For the daily routine of preparing buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.
Keywords: Renal epithelial cells - MDCK - mitochondrial pH - cytosolic pH - mitochondrial sodium - mitochondrial calcium - SNARF-1 - CoroNa Red - rhod 2 - confocal microscopy
Document URI:
Link to publication: 10.1007/978-1-60761-404-3
ISBN: 978-1-60761-405-0
DOI: 10.1007/978-1-60761-404-3
Category: B2
Type: Book Section
Appears in Collections:Research publications

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