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http://hdl.handle.net/1942/10446
Title: | Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays | Authors: | HOOYBERGHS, Jef Baiesi, Marco Ferrantini, A. Carlon, E. |
Issue Date: | 2010 | Publisher: | AMER PHYSICAL SOC | Source: | PHYSICAL REVIEW E, 81(1) | Abstract: | Test experiments of hybridization in DNA microarrays show systematic deviations from the equilibrium isotherms. We argue that these deviations are due to the presence of a partially hybridized long-lived state, which we include in a kinetic model. Experiments confirm the model predictions for the intensity vs free-energy behavior. The existence of slow relaxation phenomena has important consequences for the specificity of microarrays as devices for the detection of a target sequence from a complex mixture of nucleic acids. | Notes: | [Hooyberghs, J.] Vlaamse Instelling Technol Onderzoek, Flemish Inst Technol Res, B-2400 Mol, Belgium. [Hooyberghs, J.; Baiesi, M.; Ferrantini, A.; Carlon, E.] Katholieke Univ Leuven, Inst Theoret Phys, B-3001 Heverlee, Belgium. [Hooyberghs, J.] Hasselt Univ, B-3590 Diepenbeek, Belgium. | Document URI: | http://hdl.handle.net/1942/10446 | ISSN: | 1539-3755 | DOI: | 10.1103/PhysRevE.81.012901 | ISI #: | 000274003300099 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2011 |
Appears in Collections: | Research publications |
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