Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/11473
Title: | Proteome Analysis of Multiple Compartments in a Mouse Model of Chemical-Induced Asthma | Authors: | HAENEN, Steven Vanoirbeek, Jeroen A. J. De Vooght, Vanessa Maes, Evelyne Schools, Liliane Nemery, Benoit Hoet, Peter H. M. CLYNEN, Elke |
Issue Date: | 2010 | Publisher: | AMER CHEMICAL SOC | Source: | JOURNAL OF PROTEOME RESEARCH, 9 (11). p. 5868-5876 | Abstract: | Occupational asthma is the principal cause of work-related respiratory disease in the industrial world. Toluene-2,4-diisocyanate (TDI) is one of the most common respiratory sensitizers leading to occupational asthma. Using a mouse model of chemical-induced asthma, we explored proteome changes in multiple compartments of mice sensitized and challenged with TDI or acetone-olive oil (AOO; vehicle). Airway reactivity to methacholine and a bronchoalveolar lavage (BAL) cell count was assessed in treated and control mice, 1 day after challenge. Subsequently, two-dimensional differential gel electrophoresis (2D-DIGE) was performed on auricular lymph nodes, BAL, and serum comparing TDI-treated and vehicletreated control mice. The differentially expressed proteins were identified by mass spectrometry and pathway analysis was performed. TDI-treated mice exhibit increased airway reactivity (2.6-fold increase) and a neutrophilic inflammation in the BAL fluid, compared to control mice. 2D-DIGE showed 53, 210, and 40 differentially expressed proteins in the auricular lymph nodes, BAL, and serum of TDI-treated versus vehicle-treated mice, respectively. Several of the identified proteins could be linked with inflammation, neutrophil chemotaxis, and/or oxidative stress. Physiologic and immunologic readouts of the asthmatic phenotype, such as inflammation, were confirmed in three compartments by several of the differentially expressed proteins via 2D-DIGE and computerized pathway analysis. | Notes: | [Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Res Unit Lung Toxicol, B-3000 Louvain, Belgium. [Haenen, Steven; Maes, Evelyne; Schools, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, B-3000 Louvain, Belgium. [Clynen, Elke] Hasselt Univ, BIOMED Res Inst, B-3590 Diepenbeek, Belgium. peter.hoet@med.kuleuven.be | Keywords: | 2D-DIGE; auricular lymph nodes; BALB/c mouse; bronchoalveolar lavage; serum; toluene-2,4-diisocyanate;2D-DIGE; auricular lymph nodes; BALB/c mouse; bronchoalveolar lavage; serum; toluene-2,4-diisocyanate | Document URI: | http://hdl.handle.net/1942/11473 | ISSN: | 1535-3893 | e-ISSN: | 1535-3907 | DOI: | 10.1021/pr100638m | ISI #: | 000283810500035 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2011 |
Appears in Collections: | Research publications |
Show full item record
SCOPUSTM
Citations
10
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
10
checked on Oct 11, 2024
Page view(s)
98
checked on Jul 28, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.