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Title: Analysis of the photosynthetic apparatus in transgenic tobacco plants with altered endogenous cytokinin content: a proteomic study
Authors: CORTLEVEN, Anne 
NOBEN, Jean-Paul 
VALCKE, Roland 
Issue Date: 2011
Abstract: Background: Cytokinin is a plant hormone that plays a crucial role in several processes of plant growth and development. In recent years, major breakthroughs have been achieved in the elucidation of the metabolism, the signal perception and transduction, as well as the biological functions of cytokinin. An important activity of cytokinin is the involvement in chloroplast development and function. Although this biological function has already been known for 50 years, the exact mechanisms remain elusive. Results: To elucidate the effects of altered endogenous cytokinin content on the structure and function of the chloroplasts, chloroplast subfractions (stroma and thylakoids) from transgenic Pssu-ipt and 35S:CKX1 tobacco (Nicotiana tabacum) plants with, respectively, elevated and reduced endogenous cytokinin content were analysed using two different 2-DE approaches. Firstly, thykaloids were analysed by blue-native polyacrylamide gel electrophoresis followed by SDS-PAGE (BN/SDS-PAGE). Image analysis of the gel spot pattern thus obtained from thylakoids showed no substantial differences between wild-type and transgenic tobacco plants. Secondly, a quantitative DIGE analysis of CHAPS soluble proteins derived from chloroplast subfractions indicated significant gel spot abundance differences in the stroma fraction. Upon identification by MALDI-TOF/TOF mass spectrometry, these proteins could be assigned to the Calvin-Benson cycle and photoprotective mechanisms. Conclusion: Taken together, presented proteomic data reveal that the constitutively altered cytokinin status of transgenic plants does not result in any qualitative changes in either stroma proteins or protein complexes of thylakoid membranes of fully developed chloroplasts, while few but significant quantitative differences are observed in stroma proteins.
Notes: [Cortleven, A; Valcke, R] Hasselt Univ, Fac Sci, Lab Mol & Phys Plant Physiol, Diepenbeek, Belgium [Noben, JP] Hasselt Univ, Biomed Res Inst, Sch Life Sci, Diepenbeek, Belgium [Noben, JP] Hasselt Univ, Transnat Univ Limburg, Diepenbeek, Belgium
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e-ISSN: 1477-5956
DOI: 10.1186/1477-5956-9-33
ISI #: 000293629800001
Category: A1
Type: Journal Contribution
Validations: ecoom 2012
Appears in Collections:Research publications

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