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Title: Transcriptomics identifies differences between ultrapure non-dioxin-like polychlorinated biphenyls (PCBs) and dioxin-like PCB126 in cultured peripheral blood mononuclear cells
Authors: Wens, B.
DE BOEVER, Patrick 
Maes, M.
Hollanders, K.
Schoeters, G.
Issue Date: 2011
Source: TOXICOLOGY, 287(1-3). p. 113-123
Abstract: Polychlorinated biphenyls (PCBs) remain ubiquitously present in human lipids despite the ban on their production and use. Their presence can be chemically monitored in peripheral blood samples of the general population. We tested whether in vitro exposure to different PCB congeners induced different gene expression profiles in peripheral blood cells. We have isolated peripheral blood mononuclear cells (PBMC) from whole blood of 8 healthy individuals and exposed these cells in vitro to individual non-dioxin-like (NDL)-PCB congeners (PCB52, 138 or 180: 10 mu M) or dioxin-like (DL)-PCB congener PCB126 (1 mu M) during 18h. Differential gene expression response was measured using Agilent whole-human genome microarrays. Two-way ANOVA analysis of the data showed that both gender and PCB exposure are important factors influencing gene expression responses in blood cells. Hierarchical cluster analysis of genes influenced by PCB exposure, revealed that DL-PCB126 induced a different gene expression response compared to the NDL-PCBs. Biological interpretation of the results revealed that exposure to PCB126 induced the AhR signalg pathway, whereas the induction of nuclear receptor pathways by the NDL-PCBs was limited in blood cells. Nevertheless, molecular responses of blood cells to individual PCB congeners revealed significantly expressed genes that play a role in biological functions and processes known to be affected by PCB exposure in vivo. Observed gene expression changes in this in vitro model were found to be related to hepatotoxicity, immune and inflammatory response and disturbance of lipid and cholesterol homeostasis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Notes: [Wens, B; De Boever, P; Maes, M; Hollanders, K; Schoeters, G] Flemish Inst Technol Res VITO, Unit Environm Risk & Hlth, B-2400 Mol, Belgium [De Boever, P] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium [Schoeters, G] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium
Keywords: Polychlorinated biphenyl (PCB); Peripheral blood mononuclear cells; Gene expression analysis; Microarray
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ISSN: 0300-483X
e-ISSN: ****-****
DOI: 10.1016/j.tox.2011.06.004
ISI #: 000294196600015
Category: A1
Type: Journal Contribution
Validations: ecoom 2012
Appears in Collections:Research publications

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