Optimalisatie en miniaturisatie van een NCD-gebaseerde immunosensor

Abstract

C-reactief protein (CRP) is a significant marker for inflammatory diseases. Recent research also indicates that high CRP-concentrations in blood are a definite risk marker for cardiovascular diseases. Current standards for CRP-detection include turbidimetric and ELISA-assays. These methods are time-consuming, expensive and require educated medical personnel. An electronic antigen detection system could allow for a faster, simple and real-time determination. In this study an existing immunosensing platform will be optimalized and miniaturized. The immunosensor is based on nanocrystalline diamond (NCD), a very appealing material for biosensing purposes because of its interesting properties, namely biocompatibility, chemical inertia and the ability to change the electrical behavior through doping. A recent study proves that a NCD-based immunosensor could detect CRP with electrochemical impedance spectroscopy (EIS). This sensor will be miniaturized to a coplanar setup for future use in a