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Title: Heat-Transfer Resistance at Solid–Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA
Authors: van Grinsven, Bart 
Vanden Bon, Natalie 
Grieten, Lars 
Murib, Mohammed 
Jimenez Monroy, Kathia L. 
Janssens, Stoffel D. 
Haenen, Ken 
Schöning, Michael J.
Vermeeren, Veronique 
Ameloot, Marcel 
Michiels, Luc 
Thoelen, Ronald 
De Ceuninck, Ward 
Wagner, Patrick 
Strauven, Hannelore
Issue Date: 2012
Publisher: American Chemical Society
Source: ACS Nano, 6(3), p. 2712-2721
Abstract: In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
Keywords: DNA polymorphisms; molecular brushes; biosensors; heat-transfer resistance; impedance spectroscopy; confocal fluorescence; microscopy; nanocrystalline CVD diamond
Document URI:
ISSN: 1936-0851
e-ISSN: 1936-086X
DOI: 10.1021/nn300147e
ISI #: 000301945900089
Category: A1
Type: Journal Contribution
Validations: ecoom 2013
Appears in Collections:Research publications

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