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http://hdl.handle.net/1942/14212
Title: | Detection of DNA-Hybridization Using Interdigitated Electrodes Functionalized with Graphene | Authors: | Lanche, Ruben | Advisors: | WAGNER, Patrick INGEBRANDT, Sven |
Issue Date: | 2012 | Publisher: | tUL Diepenbeek | Abstract: | An array of four Au-IDEs was fabricated on Si/SiO2 and glass substrates. The active area of the fabricated IDEs has been functionalized with a GO layer using dielectrophoresis (DEP). The GO layer was reduced using L-Ascorbic Acid (L-AA). Finally, the surface of each rGO-IDE was functionalized with single-stranded DNA (ssDNA) molecules with perfectly matched or fully mismatched DNA-sequences to that of the complementary ssDNA (cDNA) molecules. For detection of the DNA hybridization and denaturation, the developed DNA-chip was mounted in a measurement cell and characterized by impedance spectroscopy (IS). The DNA hybridization and denaturation experiments were carried out in a differential IS setup using an impedance analyzer to eliminate the effect of disturbing factors in the sensor's signal. Changes in impedance for the sensors functionalized with perfect-matched ssDNA after a hybridization event were observed in contrast to almost no change when fully miss-matched ssDNA was used. | Notes: | master in de biomedische wetenschappen-bio-elektronica en nanotechnologie | Document URI: | http://hdl.handle.net/1942/14212 | Category: | T2 | Type: | Theses and Dissertations |
Appears in Collections: | Master theses |
Files in This Item:
File | Description | Size | Format | |
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10302762011254.pdf | 2.73 MB | Adobe PDF | View/Open |
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