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|Title:||Human dental pulp stem cells have a positive influence on neural regeneration||Authors:||MARTENS, Wendy
|Issue Date:||2012||Publisher:||WILEY-BLACKWELL||Source:||JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 6 (Special Issue, Supplement 1), p. 80-80||Abstract:||Stem cell-based therapies can be a future novel strategy to repair peripheral nerve injury, based on their multilineage differentiation potential and ability to produce and secrete neuro(trophic) factors. Recently, a possible transdifferentiation of MSC into SC have been demonstrated which had a positive effect on neuronal survival and neurite outgrowth. In this study, dental pulp stem cells (DPSC) are differentiated toward SC via a mix of growth factors. In addition the influence of secreted neurotrophic factors bt DPSC and SC-DPSC on neuronal outgrowth and survival is investigated. After 21 days of differentiation, the expression of SC-markers, GFAP, p75 and S100 was observed together with a decreased expression of nestin and Stro-1. Ultrastructurally, SC-DPSC displayed a spindle-shaped bipolar morphology with numerous organelles spread throughout the cell cytoplasm. Furthermore, collagen fibers were observed in the extracellular matrix. DPSC and SC-DPSC produced and secreted several neurotrophic factors which promoted the survival and neurite outgrowth in DRG cultures, with SC-DPSC yielding a significantly better effect than control DPSC. The results of this study indicate that DPSC are capable of differentiating towards SC. Moreover, differentiated DPSC had a better neuroprotective and neurotrophic effect than naïve stem cells suggesting that DPSC can be good candidates for cell-based therapies as treatment for peripheral nerve injuries.||Notes:||[Martens, M. W.; Bronckaers, B. A.; Hilkens, H. P.; Gervois, G. P.; Politis, P. C.; Lambrichts, L. I.] Hasselt Univ, Biomed Res Inst, Lab Funct Morphol, Hasselt, Belgium.||Keywords:||Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology; Engineering, Biomedical||Document URI:||http://hdl.handle.net/1942/14236||ISSN:||1932-6254||e-ISSN:||1932-7005||ISI #:||000308313000294||Category:||M||Type:||Journal Contribution|
|Appears in Collections:||Research publications|
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