Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/14538
Title: Monitoring of irritant gas using a whole-cell-based sensor system
Authors: BOHRN, Ulrich 
Stuetz, E.
Fuchs, K.
Fleischer, M.
Schöning, M.J.
WAGNER, Patrick 
Issue Date: 2012
Publisher: ELSEVIER SCIENCE SA
Source: SENSORS AND ACTUATORS B-CHEMICAL, 175, p. 208-217
Abstract: Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas (R)), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. (C) 2012 Elsevier B.V. All rights reserved.
Notes: [Bohrn, U.; Stuetz, E.; Fuchs, K.; Fleischer, M.] Siemens AG, Corp Res & Technol, D-81739 Munich, Germany. [Bohrn, U.; Schoening, M. J.] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany. [Bohrn, U.; Wagner, P.] Univ Hasselt, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium.
Keywords: Cell-based gas biosensor; Whole-cell sensor; A549; RPMI 2650; Respiration; Acidification; Impedance; Mid infrared spectroscopy;Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; cell-based gas biosensor; whole-cell sensor; A549; RPMI 2650; respiration; acidification; impedance; mid infrared spectroscopy
Document URI: http://hdl.handle.net/1942/14538
e-ISSN: 0925-4005
DOI: 10.1016/j.snb.2012.05.088
ISI #: 000312358700036
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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