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Title: | Monitoring of irritant gas using a whole-cell-based sensor system | Authors: | BOHRN, Ulrich Stuetz, E. Fuchs, K. Fleischer, M. Schöning, M.J. WAGNER, Patrick |
Issue Date: | 2012 | Publisher: | ELSEVIER SCIENCE SA | Source: | SENSORS AND ACTUATORS B-CHEMICAL, 175, p. 208-217 | Abstract: | Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas (R)), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. (C) 2012 Elsevier B.V. All rights reserved. | Notes: | [Bohrn, U.; Stuetz, E.; Fuchs, K.; Fleischer, M.] Siemens AG, Corp Res & Technol, D-81739 Munich, Germany. [Bohrn, U.; Schoening, M. J.] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany. [Bohrn, U.; Wagner, P.] Univ Hasselt, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium. | Keywords: | Cell-based gas biosensor; Whole-cell sensor; A549; RPMI 2650; Respiration; Acidification; Impedance; Mid infrared spectroscopy;Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; cell-based gas biosensor; whole-cell sensor; A549; RPMI 2650; respiration; acidification; impedance; mid infrared spectroscopy | Document URI: | http://hdl.handle.net/1942/14538 | e-ISSN: | 0925-4005 | DOI: | 10.1016/j.snb.2012.05.088 | ISI #: | 000312358700036 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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