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Title: Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro
Authors: BOHRN, Ulrich 
Stuetz, Evamaria
Fleischer, Maximilian
Schoening, Michael J.
WAGNER, Patrick 
Issue Date: 2013
Source: BIOSENSORS & BIOELECTRONICS, 40 (1), p. 393-400
Abstract: In this study, a cell-based gas biosensor is presented used for the detection and investigation of gaseous organic compounds in air. The response of living human nasal cells (RPMI 2650) and human lung cells (A549) towards the direct exposure of gaseous substances for 10 min is monitored with a multiparametric sensor system. Changes in the cellular impedance, oxygen consumption rate and acidification rate can be recorded after the exposure and represent the cytotoxicity of the present gas. The sensor is able to notify the presence of acetone in aqueous solution (2%) but in notably lower concentrations in the gas phase (100-333 ppm) within 30-60 min after the end of the gas exposure. Cell viability is not affected by a sequential exposure to humidified synthetic air (60% r.h.) with a flow rate of 300 ml/min and therefore offers the possibility for a continuous air monitoring. In addition, exposure to synthetic air has no influence on the signals of consecutive acetone exposure. The system might be used in the future for the monitoring of ambient air in work spaces. (C) 2012 Elsevier B.V. All rights reserved.
Notes: [Bohrn, Ulrich; Stuetz, Evamaria; Fleischer, Maximilian] Siemens AG, Corp Res & Technol, D-81739 Munich, Germany. [Bohrn, Ulrich; Schoening, Michael J.] Aachen Univ Appl Sci, INB, D-52428 Julich, Germany. [Bohrn, Ulrich; Wagner, Patrick] Hasselt Univ, Inst Mat Res IMO IMOMEC, B-3590 Diepenbeek, Belgium.
Keywords: Cell-based gas biosensor; Acetone; A549; RPMI 2650; Impedance; Respiration;Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology; cell-based gas biosensor; acetone; A549; RPMI 2650; impedance; respiration
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ISSN: 0956-5663
e-ISSN: 1873-4235
DOI: 10.1016/j.bios.2012.08.029
ISI #: 000312825800061
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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