Please use this identifier to cite or link to this item:
Title: Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco
Authors: Spoustova, P.
Synkova, H.
VALCKE, Roland 
Cerovska, N.
Issue Date: 2013
Publisher: SPRINGER
Source: PHOTOSYNTHETICA, 51 (2), p. 191-201
Abstract: The effect of Potato virus Y (NTN) (PVY) infection upon photosynthesis was analysed in transgenic Pssu-ipt tobacco overproducing endogenous cytokinins in comparison with control, nontransgenic Nicotiana tabacum plants. The course of the infection from the early to the late stage was monitored by measuring of photosynthetic gas exchange and fast chlorophyll (Chl) a fluorescence induction kinetics. Leaf photosynthesis was also analysed using Chl fluorescence imaging (Chl-FI). From the different fluorescence parameters obtained using Chl-FI, the nonphotochemical quenching (NPQ) proved to be the most useful parameter to assess the effect of PVY infection. On the other hand, Chl-FI was found to be inapplicable for any presymptomatic detection of PVY infection in tobacco. The lower accumulation of the virus was found in transgenic plants and corresponded also with the presence of visible symptoms of PVY infection. The net photosynthetic rate (P (N)), transpiration rate (E), and stomatal conductance (g (s)) significantly decreased with the progress of the infection in both control plant types and transgenic rooted plants, while transgenic grafts were much less affected. The analysis of the Chl fluorescence transient revealed higher number of silent dissipative reaction centres, higher nonphotochemical dissipation, and significantly lower performance index, PI(abs), in the healthy transgenic grafts. Chl-FI also confirmed significantly higher NPQ in transgenic grafts.
Notes: Acad Sci CR, Inst Expt Bot, CZ-16502 Prague 6, Czech Republic. Charles Univ Prague, Fac Sci, Dept Expt Biol Plants, CZ-12844 Prague 2, Czech Republic. Hasselt Univ, Fac Sci, Lab Mol & Phys Plant Physiol, B-3590 Diepenbeek, Belgium.
Keywords: Plant Sciences;chlorophyll a fluorescence imaging; cytokinins; gas-exchange parameters; ipt; photosynthesis; Potato virus Y; transgenic tobacco
Document URI:
ISSN: 0300-3604
e-ISSN: 1573-9058
DOI: 10.1007/s11099-013-0023-4
ISI #: 000318282600003
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

Show full item record


checked on Sep 3, 2020


checked on May 21, 2022

Page view(s)

checked on May 20, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.