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Title: | DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria | Authors: | GEETS, Joke Borremans, Brigitte Diels, Ludo Springael, Dirk VANGRONSVELD, Jaco VAN DER LELIE, Daniel Vanbroekhoven, Karolien |
Issue Date: | 2006 | Publisher: | Elsevier | Source: | JOURNAL OF MICROBIOLOGICAL METHODS, 66(2). p. 194-205 | Abstract: | A denaturing gradient gel electrophoresis (DGGE) method was developed to assess the diversity of dsrB (dissimilatory sulfite reductase β-subunit)-genes in sulfate-reducing communities. For this purpose a PCR primer pair was optimized for the amplification of a similar to 350 bp dsrB gene fragment that after DGGE gel electrophoresis enabled us to discriminate between dsrB genes of different SRB-subgroups,-genera and -species. The dsrB-DGGE method revealed considerable genetic diversity when applied to DNA extracts obtained from aquifer samples that were derived from monitoring wells of an in situ metal precipitation (ISMP) pilot project conducted at the site of a non-ferrous industry or from environmental heavy metal contaminated samples. The sequences of the excised and sequenced DGGE bands represented dsrB genes of different SRB-subgroups,-genera and -species, thus confirming the broad applicability of the PCR primer pair. Linking the results of the physico-chemical follow-up of the field and lab experiments to the dsrB-DGGE data will provide a better understanding of the contribution of the SRB populations to the ongoing ISMP processes. | Keywords: | sulfate-reducing bacteria; denaturing gradient gel electrophoresis (DGGE); dissimilatory sulfite reductase (dsr); environmental samples;Sulfate-reducing bacteria; Denaturing gradient gel electrophoresis (DGGE); Dissimilatory sulfite reductase (dsr); Environmental samples | Document URI: | http://hdl.handle.net/1942/1535 | ISSN: | 0167-7012 | e-ISSN: | 1872-8359 | DOI: | 10.1016/j.mimet.2005.11.002 | ISI #: | 000238963000002 | Rights: | (C) 2005 Elsevier B.V. All rights reserved | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2007 |
Appears in Collections: | Research publications |
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