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|Title:||Heat-transfer-based detection of L-nicotine, histamine, and serotonin using molecularly imprinted polymers as biomimetic receptors||Authors:||PEETERS, Marloes
VAN GRINSVEN, Bart
DE CEUNINCK, Ward
Troost, F. J.
|Issue Date:||2013||Publisher:||SPRINGER HEIDELBERG||Source:||ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 405 (20), p. 6453-6460||Abstract:||In this work, we will present a novel approach for the detection of small molecules with molecularly imprinted polymer (MIP)-type receptors. This heat-transfer method (HTM) is based on the change in heat-transfer resistance imposed upon binding of target molecules to the MIP nanocavities. Simultaneously with that technique, the impedance is measured to validate the results. For proof-of-principle purposes, aluminum electrodes are functionalized with MIP particles, and l-nicotine measurements are performed in phosphate-buffered saline solutions. To determine if this could be extended to other templates, histamine and serotonin samples in buffer solutions are also studied. The developed sensor platform is proven to be specific for a variety of target molecules, which is in agreement with impedance spectroscopy reference tests. In addition, detection limits in the nanomolar range could be achieved, which is well within the physiologically relevant concentration regime. These limits are comparable to impedance spectroscopy, which is considered one of the state-of-the-art techniques for the analysis of small molecules with MIPs. As a first demonstration of the applicability in biological samples, measurements are performed on saliva samples spiked with l-nicotine. In summary, the combination of MIPs with HTM as a novel readout technique enables fast and low-cost measurements in buffer solutions with the possibility of extending to biological samples.||Notes:||Peeters, M (reprint author); [Peeters, M.; Csipai, P.; Geerets, B.; Weustenraed, A.; van Grinsven, B.; Thoelen, R.; De Ceuninck, W.; Cleij, T. J.; Wagner, P.] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Csipai, P.; Gruber, J.] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, Brazil. [Thoelen, R.] Xios Univ, Coll Limburg, B-3590 Diepenbeek, Belgium. [De Ceuninck, W.; Wagner, P.] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Troost, F. J.] Maastricht Univ, Med Ctr, Dept Internal Med, Div Gastroenterol Hepatol, NL-6211 LK Maastricht, Netherlands. firstname.lastname@example.org||Keywords:||Biochemical Research Methods; Chemistry, Analytical;Molecularly imprinted polymers (MIPs); Heat-transfer resistance (Rth); Impedance spectroscopy; L-Nicotine; Heat-transfer method (HTM)||Document URI:||http://hdl.handle.net/1942/15454||ISSN:||1618-2642||e-ISSN:||1618-2650||DOI:||10.1007/s00216-013-7024-9||ISI #:||000321911800008||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2014|
|Appears in Collections:||Research publications|
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