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http://hdl.handle.net/1942/15510
Title: | Identification of coronin-1a as a novel antibody target for clinically isolated syndrome and multiple sclerosis | Authors: | ROUWETTE, Myrthe NOBEN, Jean-Paul VAN HORSSEN, Jack VAN WIJMEERSCH, Bart Hupperts, Raymond Jongen, Peter J. Verbeek, Marcel M. De Deyn, Peter P. STINISSEN, Piet SOMERS, Veerle |
Issue Date: | 2013 | Source: | JOURNAL OF NEUROCHEMISTRY, 126 (4), p. 483-492 | Abstract: | Recently, we identified the mimotope UH-CIS6 as a novel candidate antibody target for clinically isolated syndrome (CIS) and relapsing-remitting (RR) multiple sclerosis (MS). The purpose of this study was to further validate UH-CIS6 as an antibody target for CIS and MS and to identify the in vivo antibody target of UH-CIS6. First, a UH-CIS6 peptide ELISA was optimized. Next, we investigated the antibody response toward UH-CIS6 in cerebrospinal fluid (CSF) from patients with CIS (n = 20), MS (n = 43) and other neurological diseases (n = 42). Immunoprecipitation of anti-UH-CIS6 antibodies on a normal human brain lysate was performed to identify the in vivo antibody target of UH-CIS6. The cellular expression of an in vivo candidate target was investigated by immunohistochemistry using MS brain tissue sections. Antibody reactivity toward UH-CIS6 was detected in a significantly increased proportion of CSF samples from CIS and RR-MS patients as compared with neurological controls (p = 0.046). We identified and confirmed coronin-1a as the in vivo antibody target for UH-CIS6. Furthermore, coronin-1a was expressed by T cells and macrophages in an active MS lesion. Together, these results demonstrate that coronin-1a is a novel antibody target for CIS and MS. | Document URI: | http://hdl.handle.net/1942/15510 | ISSN: | 0022-3042 | e-ISSN: | 1471-4159 | DOI: | 10.1111/jnc.12335 | ISI #: | 000322721600007 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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