Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1594
Title: Comparative Proteome Analysis Of Proteins From Erwinia Amylovora Grown In Vitro And In Planta.
Authors: THOELEN, Mieke 
Deckers, T.
VANTHOURNOUT, Sofie 
NOBEN, Jean-Paul 
ROBBEN, Johan 
VALCKE, Roland 
Issue Date: 2006
Publisher: ISPP
Source: The 11th International Conference on Plant Pathogenic Bacteria. p. 48-49.
Abstract: Fire blight, caused by the bacterium Erwinia amylofora, affects most species of the Malaceae and some species in other families of the Rosales. The severity of the disease results from its destructive character on the one hand and the lack of effective control methods on the other hand. To gain a better insight into the infection process and proteins involved, the proteome of bacteria grown in vitro and in planta(M9 kloon 29, apple rootstocks)was studied. After two-dimensional electrophoresis, differentially regulated protein spots were excised, trypsinized and analysed by LC-ESI-MS/MS. A total of 127 differentially expressed proteins were identified of which 108 are-up regulated in planta and 19 proteins are down-regulated. Most of the identified proteins were involved in general metabolism; however 26% are related to oxidative stress. Numerous defence mechanisms against oxidative stress are strongly up-regulated in bacteria in planta. The protective mechanisms can be divided into three groups : prevention of ROS generation, detoxification of radicals and repair of damaged elements. Our hypothesis is that these protective mechanisms are one of the strategies used by Erwinia amylovora to escape plant defence mechanisms.
Keywords: Erwinia, proteomics
Document URI: http://hdl.handle.net/1942/1594
Category: C2
Type: Proceedings Paper
Appears in Collections:Research publications

Show full item record

Page view(s)

14
checked on May 25, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.