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http://hdl.handle.net/1942/1596
Title: | Differential Expression Of Proteins In Apple Following Inoculation With Erwinia Amylovora | Authors: | HEYENS, Kathleen VALCKE, Roland DUMONT, Debora ROBBEN, Johan NOBEN, Jean-Paul |
Issue Date: | 2006 | Publisher: | INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE | Source: | Bazzi, C. & Mazzucchi, U. (Ed.) PROCEEDINGS OF THE XTH INTERNATIONAL WORKSHOP ON FIRE BLIGHT. p. 489-494. | Series/Report: | ACTA HORTICULTURAE | Series/Report no.: | 704 | Abstract: | This work is a first attempt to create a protein map of apple leaf material inoculated with Erwinia amylovora. Apple leaf material was inoculated with E. amylovora or mock inoculated. Proteins were extracted from this material and 2-D gel electrophoresis (2-DE) was performed. 2-D gel spots were picked and identified by mass spectrometry. So far, 73 protein spots have been identified, amongst which proteins involved in photosynthesis and in the Calvin-Benson cycle and subunits of ATP-ase. The various 2-D gels were analyzed to identify differentially expressed proteins. Identified proteins upregulated upon E. amylovora inoculation include members of the PR-protein families 2, 5 and 10. PR-2 is the only PR protein shown to be upregulated systemically. | Keywords: | Malus, proteomics, 2D electrophoresis, fire blight, PR-proteins | Document URI: | http://hdl.handle.net/1942/1596 | ISBN: | 978-90-66054-07-3 | ISI #: | 000238489000078 | Category: | C1 | Type: | Proceedings Paper | Validations: | ecoom 2007 |
Appears in Collections: | Research publications |
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