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http://hdl.handle.net/1942/15993
Title: | Targeted resequencing of HIV variants by microarray thermodynamics | Authors: | Hadiwikarta, Wahyu W. Van Dorst, Bieke Hollanders, Karen Stuyver, Lieven Carlon, Enrico HOOYBERGHS, Jef |
Issue Date: | 2013 | Source: | NUCLEIC ACIDS RESEARCH, 41 (18), (ART N° e173) | Abstract: | Within a single infected individual, a virus population can have a high genomic variability. In the case of HIV, several mutations can be present even in a small genomic window of 20-30 nucleotides. For diagnostics purposes, it is often needed to resequence genomic subsets where crucial mutations are known to occur. In this article, we address this issue using DNA microarrays and inputs from hybridization thermodynamics. Hybridization signals from multiple probes are analysed, including strong signals from perfectly matching (PM) probes and a large amount of weaker cross-hybridization signals from mismatching (MM) probes. The latter are crucial in the data analysis. Seven coded clinical samples (HIV-1) are analyzed, and the microarray results are in full concordance with Sanger sequencing data. Moreover, the thermodynamic analysis of microarray signals resolves inherent ambiguities in Sanger data of mixed samples and provides additional clinically relevant information. These results show the reliability and added value of DNA microarrays for point-of-care diagnostic purposes. | Notes: | Hooyberghs, J (reprint author), VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. jef.hooyberghs@vito.be | Document URI: | http://hdl.handle.net/1942/15993 | ISSN: | 0305-1048 | e-ISSN: | 1362-4962 | DOI: | 10.1093/nar/gkt682 | ISI #: | 000325776600004 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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hadiwikarta 1.pdf | Published version | 495.2 kB | Adobe PDF | View/Open |
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