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Title: The need for transparency and good practices in the qPCR literature
Authors: Bustin, Stephen A.
Benes, Vladimir
Garson, Jeremy
Hellemans, Jan
Huggett, Jim
Kubista, Mikael
Mueller, Reinhold
Nolan, Tania
Pfaffl, Michael W.
Shipley, Gregory
Wittwer, Carl T.
Schjerling, Peter
Day, Philip J.
Abreu, Monica
Aguado, Begona
Beaulieu, Jean-Francois
Beckers, Anneleen
Bogaert, Sara
Browne, John A.
Carrasco-Ramiro, Fernando
Ceelen, Liesbeth
Ciborowski, Kate
Cornillie, Pieter
Coulon, Stephanie
De Brouwer, Sara
De Ceuninck, Leentje
De Craene, Jurgen
De Naeyer, Helene
De Spiegelaere, Ward
Deckers, Kato
Dheedene, Annelies
Durinck, Kaat
Ferreira-Teixeira, Margarida
Fieuw, Annelies
Gallup, Jack M.
Gonzalo-Flores, Sandra
Goossens, Karen
Heindryckx, Femke
Herring, Elizabeth
Hoenicka, Hans
Icardi, Laura
Jaggi, Rolf
Javad, Farzad
Karampelias, Michael
Kibenge, Frederick
Kibenge, Molly
Kumps, Candy
Lambertz, Irina
Lammens, Tim
Markey, Amelia
Messiaen, Peter
Mets, Evelien
Morais, Sofia
Mudarra-Rubio, Alberto
Nakiwala, Justine
Nelis, Hilde
Olsvik, Pal A.
Perez-Novo, Claudina
PLUSQUIN, Michelle 
Rihani, Ali
Rodrigues-Santos, Paulo
Rondou, Pieter
Sanders, Rebecca
Schmidt-Bleek, Katharina
Skovgaard, Kerstin
SMEETS, Karen 
Tabera, Laura
Toegel, Stefan
Van Acker, Tim
Van den Broeck, Wim
Van der Meulen, Joni
Van Gele, Mireille
Van Peer, Gert
Van Poucke, Mario
Van Roy, Nadine
Vergult, Sarah
Wauman, Joris
Tshuikina-Wiklander, Marina
Willems, Erik
Zaccara, Sara
Zeka, Fjoralba
Vandesompele, Jo
Issue Date: 2013
Source: NATURE METHODS, 10 (11), p. 1063-1067
Abstract: Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
Notes: Bustin, SA (reprint author), Anglia Ruskin Univ, Postgrad Med Inst, Chelmsford, Essex, England.
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ISSN: 1548-7091
e-ISSN: 1548-7105
DOI: 10.1038/nmeth.2697
ISI #: 000326507600016
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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