Title: | The need for transparency and good practices in the qPCR literature |
Authors: | Bustin, Stephen A. Benes, Vladimir Garson, Jeremy Hellemans, Jan Huggett, Jim Kubista, Mikael Mueller, Reinhold Nolan, Tania Pfaffl, Michael W. Shipley, Gregory Wittwer, Carl T. Schjerling, Peter Day, Philip J. Abreu, Monica Aguado, Begona Beaulieu, Jean-Francois Beckers, Anneleen Bogaert, Sara Browne, John A. Carrasco-Ramiro, Fernando Ceelen, Liesbeth Ciborowski, Kate Cornillie, Pieter Coulon, Stephanie CUYPERS, Ann De Brouwer, Sara De Ceuninck, Leentje De Craene, Jurgen De Naeyer, Helene De Spiegelaere, Ward Deckers, Kato Dheedene, Annelies Durinck, Kaat Ferreira-Teixeira, Margarida Fieuw, Annelies Gallup, Jack M. Gonzalo-Flores, Sandra Goossens, Karen Heindryckx, Femke Herring, Elizabeth Hoenicka, Hans Icardi, Laura Jaggi, Rolf Javad, Farzad Karampelias, Michael Kibenge, Frederick Kibenge, Molly Kumps, Candy Lambertz, Irina Lammens, Tim Markey, Amelia MESSIAEN, Peter Mets, Evelien Morais, Sofia Mudarra-Rubio, Alberto Nakiwala, Justine Nelis, Hilde Olsvik, Pal A. Perez-Novo, Claudina PLUSQUIN, Michelle REMANS, Tony Rihani, Ali Rodrigues-Santos, Paulo Rondou, Pieter Sanders, Rebecca Schmidt-Bleek, Katharina Skovgaard, Kerstin SMEETS, Karen Tabera, Laura Toegel, Stefan Van Acker, Tim Van den Broeck, Wim Van der Meulen, Joni Van Gele, Mireille Van Peer, Gert Van Poucke, Mario Van Roy, Nadine Vergult, Sarah Wauman, Joris Tshuikina-Wiklander, Marina Willems, Erik Zaccara, Sara Zeka, Fjoralba Vandesompele, Jo |
Issue Date: | 2013 |
Source: | NATURE METHODS, 10 (11), p. 1063-1067 |
Abstract: | Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not. |
Notes: | Bustin, SA (reprint author), Anglia Ruskin Univ, Postgrad Med Inst, Chelmsford, Essex, England.
stephen.bustin@anglia.ac.uk |
Document URI: | http://hdl.handle.net/1942/16030 |
ISSN: | 1548-7091 |
e-ISSN: | 1548-7105 |
DOI: | 10.1038/nmeth.2697 |
ISI #: | 000326507600016 |
Category: | A1 |
Type: | Journal Contribution |
Validations: | ecoom 2015 |
Appears in Collections: | Research publications
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