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Title: Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy
Authors: Martens, Thomas F.
Vercauteren, Dries
Forier, Katrien
Deschout, Hendrik
Remaut, Katrien
AMELOOT, Marcel 
Engbersen, Johan F. J.
Demeester, Jo
De Smedt, Stefaan C.
Braeckmans, Kevin
Issue Date: 2013
Source: NANOMEDICINE, 8 (12), p. 1955-1968
Abstract: Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration. Original submitted 20 April 2012; Revised submitted 22 November 2012
Notes: De Smedt, SC (reprint author), De Smedt, SC (reprint author) De Smedt, SC (reprint author)
Keywords: diffusion; intravitreal injection; nanomedicine; nanoparticle; ocular gene therapy; polyplex; single-particle tracking
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ISSN: 1743-5889
e-ISSN: 1748-6963
DOI: 10.2217/nnm.12.202
ISI #: 000327379600009
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

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