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http://hdl.handle.net/1942/16078
Title: | Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy | Authors: | Martens, Thomas F. Vercauteren, Dries Forier, Katrien Deschout, Hendrik Remaut, Katrien PAESEN, Rik AMELOOT, Marcel Engbersen, Johan F. J. Demeester, Jo De Smedt, Stefaan C. Braeckmans, Kevin |
Issue Date: | 2013 | Source: | NANOMEDICINE, 8 (12), p. 1955-1968 | Abstract: | Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration. Original submitted 20 April 2012; Revised submitted 22 November 2012 | Notes: | De Smedt, SC (reprint author), De Smedt, SC (reprint author) De Smedt, SC (reprint author) | Keywords: | diffusion; intravitreal injection; nanomedicine; nanoparticle; ocular gene therapy; polyplex; single-particle tracking | Document URI: | http://hdl.handle.net/1942/16078 | ISSN: | 1743-5889 | e-ISSN: | 1748-6963 | DOI: | 10.2217/nnm.12.202 | ISI #: | 000327379600009 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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