Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16095
Title: reMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process.
Authors: VANBRABANT, Jeroen 
LEIRS, Karen
VANSCHOENBEEK, Katrijn 
LAMMERTYN, Jeroen
MICHIELS, Luc 
Issue Date: 2014
Source: ANALYST, 139 (3), p. 589-595
Abstract: Current aptamer selection procedures enable limited control and transparency on how the DNA selection pool is evolving. Affinity tests and binding analysis are not always as informative. Here we show that real-time PCR provides a valuable tool for the follow-up of aptamer selection. Limited time, work and amount of amplified ssDNA make this an interesting instrument to set-up a SELEX design and monitor the enrichment of oligonucleotides. reMelting Curve Analysis (rMCA) after reannealing at stringent conditions provides information about enrichment, compared to a random library. Monitoring the SELEX process and optimizing conditions by means of the proposed methods can increase the selection efficiency in a controlled way. rMCA is applied in enrichment simulations and three different selection procedures. Our results imply that rMCA can be used for different SELEX designs and different targets. SELEX pool diversity analysis by rMCA is proven to be a useful, reproducible tool to detect and evaluate enrichment of specific binding aptamers while the selection procedure is performed.
Notes: Vanbrabant, J (reprint author),Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium, 1jeroenvanbrabant@gmail.com; luc.michiels@uhasselt.be
Keywords: Aptamers; SELEX; enrichment monitoring; real-time PCR
Document URI: http://hdl.handle.net/1942/16095
Link to publication: http://pubs.rsc.org/en/content/articlelanding/2013/an/c3an01884a#!divAbstract
ISSN: 0003-2654
e-ISSN: 1364-5528
DOI: 10.1039/C3AN01884A
ISI #: 000328912000009
Rights: © Royal Society of Chemistry 2014
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

Files in This Item:
File Description SizeFormat 
J. Vanbrabant et al - 2014 - rMCA as a tool for enrichment monitoring in the SELEX process.pdfMain article702.19 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

20
checked on Sep 3, 2020

WEB OF SCIENCETM
Citations

23
checked on May 21, 2022

Page view(s)

58
checked on May 21, 2022

Download(s)

220
checked on May 21, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.