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http://hdl.handle.net/1942/16135
Title: | Sortase A-mediated functionalization of nanobodies toward surface coupling | Authors: | TA, Duy Tien STEEN REDEKER, Erik GUEDENS, Wanda ADRIAENSENS, Peter |
Issue Date: | 2013 | Source: | Engineering of Functional Interfaces 2013, Hasselt, 8-9/7/2013 | Abstract: | The conserved LPETG motif, at which sortase A-catalyzed transpeptidation occurs, is engineered at the C-terminal region of the variable domain of single-domain heavy chain antibody (or nanobody) against Vascular Cell Adhesion Molecule 1 (VCAM1). The recombinant nanobody can then subsequently be ligated, under sortase A catalysis, to a variety of oligoglycine containing targets, including material surfaces, contrast labeling molecules and molecules functionalized for specific chemical coupling reactions. | Keywords: | nanobody, sortase A, surface coupling, VCAM1 | Document URI: | http://hdl.handle.net/1942/16135 | Category: | C2 | Type: | Conference Material |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Poster_Tien_final.pdf | Conference material | 240.53 kB | Adobe PDF | View/Open |
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