Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16192
Title: Determination of PCDD/Fs, PBDD/Fs and dioxin-like PCBs in human milk from mothers residing in the rural areas in Flanders, using the CALUX bioassay and GC-HRMS
Authors: CROES, Kristof 
Colles, A.
Koppen, G.
De Galan, S.
Vandermarken, T.
Govarts, E.
BRUCKERS, Liesbeth 
Nelen, V.
Schoeters, G.
Van Larebeke, N.
Denison, M. S.
Mampaey, M.
Baeyens, W.
Issue Date: 2013
Publisher: ELSEVIER SCIENCE BV
Source: TALANTA, 113, p. 99-105
Abstract: Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the determination of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a new CALUX method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in small amounts of human milk samples with the new sensitive H1L7.5c1 cell line was used to analyze 84 human milk samples, collected from mothers residing in the Flemish rural communities. The geometric mean CALUX-Bioanalytical Equivalent (CALUX-BEQ) values, reported for the 84 mothers from the study area were 10.4 (95% Cl: 9.4-11.4) pg CALUX-BEQ per gram lipid or 0.41 (95% Cl: 0.37-0.45) pg CALUX-BEQ per gram milk for the PCDD/Fs and 1.73 (1.57-1.91) pg CALUX-BEQ per gram lipid or 0.07 (95% CI: 0.06-0.08) pg CALUX-BEQ per gram milk for the dioxin-like PCBs. Multiple regression analysis showed significant associations between PCDD/Fs and weight change after pregnancy, smoking and consumption of local eggs. One pooled human milk sample was analyzed with both CALUX and GC-HRMS. The ratio of CALUX and GC-HRMS results for this sample were respectively 1.60, 0.58 and 1.23 for the PCDD/Fs, the dl-PCBs and the sum of both fractions, when using the 2005-TEF values. Additionally, also low levels of certain brominated dioxins and furans were detected in the pooled sample with GC-HRMS. (C) 2013 Elsevier B.V. All rights reserved.
Notes: [Croes, K.; De Galan, S.; Vandermarken, T.; Baeyens, W.] Vrije Univ Brussel, Dept Analyt & Environm Chem ANCH, B-1050 Brussels, Belgium. [Colles, A.; Koppen, G.; Govarts, E.; Schoeters, G.] Vlaamse Instelling Technol Onderzoek, B-2400 Mol, Belgium. [Bruckers, L.] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, B-3500 Hasselt, Belgium. [Nelen, V.] Prov Inst Hyg, B-2000 Antwerp, Belgium. [Croes, K.; Van Larebeke, N.] Ghent Univ Hosp, Study Ctr Carcinogenesis & Primary Prevent Canc, B-9000 Ghent, Belgium. [Denison, M. S.] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA. [Mampaey, M.] Minist Flemish Community, Flemish Environm Nat & Energy Dept LNE, B-1000 Brussels, Belgium.
Keywords: Chemistry; Analytical; PCDD/Fs; Dioxin-likePCBs; PBDD/Fs; CALUX; GC-HRMS; Human milk;PCDD/Fs; Dioxin-like PCBs; PBDD/Fs; CALUX; GC-HRMS; Human milk
Document URI: http://hdl.handle.net/1942/16192
ISSN: 0039-9140
e-ISSN: 1873-3573
DOI: 10.1016/j.talanta.2013.03.086
ISI #: 000320416500015
Category: A1
Type: Journal Contribution
Validations: ecoom 2014
Appears in Collections:Research publications

Files in This Item:
File Description SizeFormat 
croes 1.txt
  Restricted Access
Published version7.25 kBTextView/Open    Request a copy
Show full item record

SCOPUSTM   
Citations

16
checked on Sep 2, 2020

WEB OF SCIENCETM
Citations

33
checked on Mar 22, 2024

Page view(s)

62
checked on Sep 7, 2022

Download(s)

6
checked on Sep 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.