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Title: | Autoantigen induced clonal expansion in immortalized B cells from the peripheral blood of multiple sclerosis patients | Authors: | FRAUSSEN, Judith Vrolix, Kathleen CLAES, Nele Martinez-Martinez, Pilar Losen, Mario Hupperts, Raymond VAN WIJMEERSCH, Bart Espiño, Mercedes Villar, Luisa M. DE BAETS, Marc STINISSEN, Piet SOMERS, Veerle |
Issue Date: | 2013 | Source: | JOURNAL OF NEUROIMMUNOLOGY, 261 (1-2), p. 98-107 | Abstract: | We studied Ig heavy chain (VDJ) sequences and antigen reactivity of 412 immortalized B cell lines from the peripheral blood of 10 multiple sclerosis (MS) patients, 4 clinically isolated syndrome (CIS) patients and 6 healthy controls (HCs). 78/238 (32.8%) MS and CIS B cell lines were part of 9 clonally expanded B cell populations, of which 5 were present in multiple patients. Increased VH1 gene family usage was evidenced for MS B cells, with 29.2% expressing VH1-69. Affinity maturation in MS and CIS was indicated by increased Ig VDJ mutations. Autoantibody producing B cells reactive to intracellular antigens were significantly higher in MS (25%) and CIS (28%) patients than in HCs (5%), including 3/9 expanded B cell clones. Specificity for phosphatidylcholine was observed for 1/9 B cell clones. These findings indicate clonally expanded autoreactive B cells with affinity maturation in the peripheral blood in MS and CIS | Keywords: | Autoreactivity; B cell immortalization; Clinically isolated syndrome; Clonal B cell expansion; Complementarity determining region 3; Multiple sclerosis | Document URI: | http://hdl.handle.net/1942/16342 | ISSN: | 0165-5728 | e-ISSN: | 1872-8421 | DOI: | 10.1016/j.jneuroim.2013.05.002 | ISI #: | 000323459400014 | Rights: | © 2013 Elsevier B.V. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2014 |
Appears in Collections: | Research publications |
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