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http://hdl.handle.net/1942/16684
Title: | An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles | Authors: | Subramanian, Palaniappan Motorina, Anastasiia YEAP, Weng Siang HAENEN, Ken Coffinier, Yannick Zaitsev, Vladimir Niedziolka-Jonsson, Joanna Boukherroub, Rabah Szunerits, Sabine |
Issue Date: | 2014 | Source: | ANALYST, 139 (7), p. 1726-1731 | Abstract: | Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL−1 (2 nM) with a dynamic range up to 300 ng mL−1 (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution. | Notes: | Szunerits, S (reprint author), Univ Lille 1, CNRS USR 3078, IRI, Parc Haute Borne,50 Ave Halley,BP 70478, F-59658 Villeneuve Dascq, France. | Document URI: | http://hdl.handle.net/1942/16684 | ISSN: | 0003-2654 | e-ISSN: | 1364-5528 | DOI: | 10.1039/c3an02045b | ISI #: | 000332422400021 | Rights: | This journal is © The Royal Society of Chemistry 2014 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
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