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|Title:||Analysis of alpha 3 GlyR single particle tracking in the cell membrane||Authors:||NOTELAERS, Kristof
DE CLERCQ, Ben
Meier, Jochen C.
|Issue Date:||2014||Source:||BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1843 (3), p. 544-553||Abstract:||Single particle tracking (SPT) of transmembrane receptors in the plasma membrane often reveals heterogeneous diffusion. A thorough interpretation of the displacements requires an extensive analysis suited for discrimination of differentmotion types present in the data. Here the diffusion pattern of the homomeric α3-containing glycine receptor (GlyR) is analyzed in themembrane of HEK 293 cells. More specifically, the influence of the α3 RNA splice variants α3K and α3L on lateralmembrane diffusion of the receptor is revealed in detail. Using a combination of ensemble and local SPT analysis, free and anomalous diffusion parameters are determined. The GlyR α3 free diffusion coefficient is found to be 0.13 ± 0.01 μm2/s and both receptor variants display confined motion. The confinement probability level and residence time are significantly elevated for the α3L variant compared to the α3K variant. Furthermore, for the α3L GlyR, the presence of directed motion was also established, with a velocity matching that of saltatory vesicular transport. These findings reveal that α3 GlyRs are prone to different types of anomalous diffusion and reinforce the role of RNA splicing in determining lateral membrane trafficking.||Notes:||Ameloot, M (reprint author), Agoralaan Gebouw C, B-3590 Diepenbeek, Belgium. email@example.com||Keywords:||glycine receptor; alpha3 subunit; RNA splicing; single particle tracking; confined motion; directed motion||Document URI:||http://hdl.handle.net/1942/16693||ISSN:||0167-4889||e-ISSN:||1879-2596||DOI:||10.1016/j.bbamcr.2013.11.019||ISI #:||000330821000006||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2015|
|Appears in Collections:||Research publications|
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