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http://hdl.handle.net/1942/1682
Title: | Two-dimensional Forster resonance energy transfer (2-D FRET) and the membrane raft hypothesis | Authors: | ACASANDREI, Maria DALE, Robert VAN DE VEN, Martin AMELOOT, Marcel |
Issue Date: | 2006 | Publisher: | Elsevier | Source: | CHEMICAL PHYSICS LETTERS, 419(4-6). p. 469-473 | Abstract: | A model for analyzing Forster resonance energy transfer (FRET) data in relation to the cell plasma membrane raft hypothesis is developed to take into account: (a) the distribution of FRET donors and acceptors at the surface of probing antibody fragments specific for a putative raft component; (b) partitioning of the raft component between raft and non-raft areas of the membrane; and (c) the dependence of the raft partition on the expression level of the considered component. Analysis of relevant FRET data in the literature according to this model provides support for the raft hypothesis. | Keywords: | GPI-ANCHORED PROTEINS; LIPID RAFTS; MDCK CELLS; PLASMA-MEMBRANE; 2; DIMENSIONS; SURFACE; MICRODOMAINS; ORGANIZATION; DOMAINS; MICROSCOPY | Document URI: | http://hdl.handle.net/1942/1682 | ISSN: | 0009-2614 | e-ISSN: | 1873-4448 | DOI: | 10.1016/j.cplett.2005.11.104 | ISI #: | 000235675000032 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2007 |
Appears in Collections: | Research publications |
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twodimensional.pdf | Peer-reviewed author version | 159.02 kB | Adobe PDF | View/Open |
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