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Title: | Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system. | Authors: | Cambré, A. Zimmerman, M. Sauer, U. Vivijs, B. Cenens, W. Michiels, C.W. Aertsen, A. Loessner, M.J. NOBEN, Jean-Paul Ayala, J.A. Lavigne, R. Briers, Y. |
Issue Date: | 2015 | Source: | ENVIRONMENTAL MICROBIOLOGY, 17 (5), p. 1586-1599 | Abstract: | Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and ‘omics’ technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E. coli L-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labeled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are three- and four-fold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation. | Notes: | Briers, Y (reprint author), Katholieke Univ Leuven, Lab Gene Technol, Dept Biosyst, B-3001 Heverlee, Belgium. yves.briers@kuleuven.be | Document URI: | http://hdl.handle.net/1942/17110 | ISSN: | 1462-2912 | e-ISSN: | 1462-2920 | DOI: | 10.1111/1462-2920.12594 | ISI #: | 000353507100010 | Rights: | This article is protected by copyright. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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