Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17131
Title: Array Formatting of the Heat-Transfer Method (HTM) for the Detection of Small Organic Molecules by Molecularly Imprinted Polymers
Authors: Wackers, Gideon
VANDENRYT, Thijs 
Cornelis, Peter
KELLENS, Evelien 
THOELEN, Ronald 
DE CEUNINCK, Ward 
LOSADA-PEREZ, Patricia 
VAN GRINSVEN, Bart 
PEETERS, Marloes 
WAGNER, Patrick 
Issue Date: 2014
Publisher: MDPI AG
Source: SENSORS, 14 (6), p. 11016-11030
Abstract: In this work we present the first steps towards a molecularly imprinted polymer (MIP)-based biomimetic sensor array for the detection of small organic molecules via the heat-transfer method (HTM). HTM relies on the change in thermal resistance upon binding of the target molecule to the MIP-type receptor. A flow-through sensor cell was developed, which is segmented into four quadrants with a volume of 2.5 mu L each, allowing four measurements to be done simultaneously on a single substrate. Verification measurements were conducted, in which all quadrants received a uniform treatment and all four channels exhibited a similar response. Subsequently, measurements were performed in quadrants, which were functionalized with different MIP particles. Each of these quadrants was exposed to the same buffer solution, spiked with different molecules, according to the MIP under analysis. With the flow cell design we could discriminate between similar small organic molecules and observed no significant cross-selectivity. Therefore, the MIP array sensor platform with HTM as a readout technique, has the potential to become a low-cost analysis tool for bioanalytical applications.
Notes: De Ceuninck, W (reprint author), [Wackers, Gideon; Vandenryt, Thijs; Cornelis, Peter; Kellens, Evelien; Thoelen, Ronald; De Ceuninck, Ward; Losada-Perez, Patricia; van Grinsven, Bart; Peeters, Marloes; Wagner, Patrick] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Kellens, Evelien; Thoelen, Ronald; De Ceuninck, Ward; Losada-Perez, Patricia; van Grinsven, Bart; Peeters, Marloes; Wagner, Patrick] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [van Grinsven, Bart] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands. gideon.wackers@student.uhasselt.be; thijs.vandenryt@uhasselt.be; peter.cornelis@student.uhasselt.be; evelien.kellens@uhasselt.be; ronald.thoelen@uhasselt.be; Ward.Deceuninck@uhasselt.be; patricia.losadaperez@uhasselt.be; bart.vangrinsven@maastrichtuniversity.nl; marloes.peeters@uhasselt.be; patrick.wagner@uhasselt.be
Keywords: heat-transfer method (HTM); molecularly imprinted polymers (MIPs); serotonin; histamine; L-nicotine; array format;heat-transfer method (HTM); molecularly imprinted polymers (MIPs); serotonin; histamine; L-nicotine; array format
Document URI: http://hdl.handle.net/1942/17131
e-ISSN: 1424-8220
DOI: 10.3390/s140611016
ISI #: 000338635600082
Rights: © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Category: A1
Type: Journal Contribution
Validations: ecoom 2015
Appears in Collections:Research publications

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