Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17802
Title: Toxicity profiles and solvent-toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide (DMSO) exposure
Authors: STEVENS, An-Sofie 
PIROTTE, Nicky 
PLUSQUIN, Michelle 
Willems, Maxime
NEYENS, Thomas 
ARTOIS, Tom 
SMEETS, Karen 
Issue Date: 2014
Source: JOURNAL OF APPLIED TOXICOLOGY, 35 (3), p. 319-326
Abstract: To investigate hydrophobic test compounds in toxicological studies, solvents like dimethylsulfoxide (DMSO) are inevitable. However, using these solvents, the interpretation of test compound-induced responses can be biased. DMSO concentration guidelines are available, but are mostly based on acute exposures involving one specific toxicity endpoint. Hence, to avoid solvent–toxicant interference, we use multiple chronic test endpoints for additional interpretation of DMSO concentrations and propose a statistical model to assess possible synergistic, antagonistic or additive effects of test compounds and their solvents. In this study, the effects of both short- (1 day) and long-term (2 weeks) exposures to low DMSO concentrations (up to 1000 µl l−1) were studied in the planarian Schmidtea mediterranea. We measured different biological levels in both fully developed and developing animals. In a long-term exposure set-up, a concentration of 500 µl l−1 DMSO interfered with processes on different biological levels, e.g. behaviour, stem cell proliferation and gene expression profiles. After short exposure times, 500 µl l−1 DMSO only affected motility, whereas the most significant changes on different parameters were observed at a concentration of 1000 µl l−1 DMSO. As small sensitivity differences exist between biological levels and developmental stages, we advise the use of this solvent in concentrations below 500 µl l−1 in this organism. In the second part of our study, we propose a statistical approach to account for solvent–toxicant interactions and discuss full-scale solvent toxicity studies. In conclusion, we reassessed DMSO concentration limits for different experimental endpoints in the planarian S. mediterranea. Copyright © 2014 John Wiley & Sons, Ltd.
Notes: Correspondence to: Karen Smeets, Zoology: Biodiversity and Toxicology, Centre for Environmental Sciences, Hasselt University, Agoralaan, building D, BE 3590 Diepenbeek, Belgium. karen.smeets@uhasselt.be E-mail: karen.smeets@uhasselt.be
Keywords: planarians; DMSO; toxicology; different biological levels; solvent–toxicant interference
Document URI: http://hdl.handle.net/1942/17802
ISSN: 0260-437X
e-ISSN: 1099-1263
DOI: 10.1002/jat.3011
ISI #: 000349370600012
Rights: Copyright © 2014 John Wiley & Sons, Ltd.
Category: A1
Type: Journal Contribution
Validations: ecoom 2016
Appears in Collections:Research publications

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