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Title: | Variation of DNA methylation in candidate age-related targets on the mitochondrial-telomere axis in cord blood and placenta | Authors: | JANSSEN, Bram Byun, H. M. COX, Bianca GYSELAERS, Wilfried Izzi, B. Baccarelli, Andrea A. NAWROT, Tim |
Issue Date: | 2014 | Source: | PLACENTA, 35 (9), p. 665-672 | Abstract: | Background: Epigenetics is tissue-specific and potentially even cell-specific, but little information is available from human reproductive studies about the concordance of DNA methylation patterns in cord blood and placenta, as well as within-placenta variations. We evaluated methylation levels at promoter regions of candidate genes in biological ageing pathways (SIRT1, TP53, PPARG, PPARGC1A, and TFAM), a subtelomeric region (D4Z4) and the mitochondrial genome (MT-RNR1, D-loop). Methods: Ninety individuals were randomly chosen from the ENVIRONAGE birth cohort to evaluate methylation concordance between cord blood and placenta using highly quantitative bisulfite-PCR pyrosequencing. In a subset of nineteen individuals, a more extensive sampling scheme was performed to examine within-placenta variation. Results: The DNA methylation levels of the subtelomeric region and mitochondrial genome showed concordance between cord blood and placenta with correlation coefficients ranging from r = 0.31 to 0.43, p <= 0.005, and also between the maternal and foetal sides of placental tissue (r = 0.53 to 0.72, p <= 0.05). For the majority of targets, an agreement in methylation levels between four foetal biopsies was found (with intra-class correlation coefficients ranging from 0.16 to 0.72), indicating small within-placenta variation. Conclusions: The methylation levels of the subtelomeric region (D4Z4) and mitochondrial genome (MT-RNR1, D-loop) showed concordance between cord blood and placenta, suggesting a common epigenetic signature of these targets between tissues. Concordance was lacking between the other genes that were studied. In placental tissue, methylation patterns of most targets on the mitochondrial-telomere axis were not strongly influenced by sample location. | Notes: | Nawrot, TS (reprint author), Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium.bram.janssen@uhasselt.be; tim.nawrot@uhasselt.be | Keywords: | ageing; cord blood; concordance; DNA methylation; epigenetics; placenta; within-placenta variation | Document URI: | http://hdl.handle.net/1942/17874 | ISSN: | 0143-4004 | e-ISSN: | 1532-3102 | DOI: | 10.1016/j.placenta.2014.06.371 | ISI #: | 000342961400001 | Rights: | © 2014 Elsevier Ltd. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2015 |
Appears in Collections: | Research publications |
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