Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/18141
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | REMANS, Tony | - |
dc.contributor.author | KEUNEN, Els | - |
dc.contributor.author | BEX, Geert Jan | - |
dc.contributor.author | SMEETS, Karen | - |
dc.contributor.author | VANGRONSVELD, Jaco | - |
dc.contributor.author | CUYPERS, Ann | - |
dc.date.accessioned | 2015-01-21T13:34:39Z | - |
dc.date.available | 2015-01-21T13:34:39Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | PLANT CELL, 26 (10), p. 3829-3837 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.uri | http://hdl.handle.net/1942/18141 | - |
dc.description.abstract | Reverse transcription-quantitative PCR (RT-qPCR) has been widely adopted to measure differences in mRNA levels; however, biological and technical variation strongly affects the accuracy of the reported differences. RT-qPCR specialists have warned that, unless researchers minimize this variability, they may report inaccurate differences and draw incorrect biological conclusions. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines describe procedures for conducting and reporting RT-qPCR experiments. The MIQE guidelines enable others to judge the reliability of reported results; however, a recent literature survey found low adherence to these guidelines. Additionally, even experiments that use appropriate procedures remain subject to individual variation that statistical methods cannot correct. For example, since ideal reference genes do not exist, the widely used method of normalizing RT-qPCR data to reference genes generates background noise that affects the accuracy of measured changes in mRNA levels. However, current RT-qPCR data reporting styles ignore this source of variation. In this commentary, we direct researchers to appropriate procedures, outline a method to present the remaining uncertainty in data accuracy, and propose an intuitive way to select reference genes to minimize uncertainty. Reporting the uncertainty in data accuracy also serves for quality assessment, enabling researchers and peer reviewers to confidently evaluate the reliability of gene expression data. | - |
dc.language.iso | en | - |
dc.rights | © 2014 American Society of Plant Biologists. All rights reserved. | - |
dc.title | Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy. | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 3837 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 3829 | - |
dc.identifier.volume | 26 | - |
local.bibliographicCitation.jcat | A2 | - |
local.type.refereed | Refereed | - |
local.type.specified | Editorial Material | - |
dc.identifier.doi | 10.1105/tpc.114.130641 | - |
dc.identifier.isi | 000345920900005 | - |
item.contributor | REMANS, Tony | - |
item.contributor | KEUNEN, Els | - |
item.contributor | BEX, Geert Jan | - |
item.contributor | SMEETS, Karen | - |
item.contributor | VANGRONSVELD, Jaco | - |
item.contributor | CUYPERS, Ann | - |
item.fullcitation | REMANS, Tony; KEUNEN, Els; BEX, Geert Jan; SMEETS, Karen; VANGRONSVELD, Jaco & CUYPERS, Ann (2014) Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy.. In: PLANT CELL, 26 (10), p. 3829-3837. | - |
item.accessRights | Open Access | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 1040-4651 | - |
crisitem.journal.eissn | 1532-298X | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Plant Cell-2014-Remans-3829-37.pdf | 560.56 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
62
checked on Sep 3, 2020
WEB OF SCIENCETM
Citations
89
checked on Apr 23, 2024
Page view(s)
128
checked on Jun 16, 2023
Download(s)
176
checked on Jun 16, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.