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http://hdl.handle.net/1942/18494
Title: | ALTERNATIVE OXIDASE1a modulates the oxidative challenge during moderate Cd exposure in Arabidopsis thaliana leaves | Authors: | KEUNEN, Els SCHELLINGEN, Kerim Van Der Straeten, Dominique REMANS, Tony COLPAERT, Jan VANGRONSVELD, Jaco CUYPERS, Ann |
Issue Date: | 2015 | Source: | JOURNAL OF EXPERIMENTAL BOTANY 66(10), p. 2967-2977 | Abstract: | This study aims to unravel the functional significance of alternative oxidase1a (AOX1a) induction in Arabidopsis thaliana leaves exposed to cadmium (Cd) by comparing wild-type (WT) plants and aox1a knockout mutants. In the absence of AOX1a, differences in stress-responsive transcript and glutathione levels suggest an increased oxidative challenge during moderate (5 µM) and prolonged (72h) Cd exposure. Nevertheless, aox1a knockout leaves showed lower hydrogen peroxide (H2O2) accumulation as compared to the WT due to both acute (24h) and prolonged (72h) exposure to 5 µM Cd, but not to 10 µM Cd. Taken together, we propose a working model where AOX1a acts early in the response to Cd and activates or maintains a mitochondrial signalling pathway impacting on cellular antioxidative defence at the post-transcriptional level. This fine-tuning pathway is suggested to function during moderate (5 µM) Cd exposure while being overwhelmed during more severe (10 µM) Cd stress. Within this framework, ethylene is required – either directly or indirectly via NADPH oxidase isoform C – to fully induce AOX1 expression. In addition, reciprocal crosstalk between these components was demonstrated in leaves of A. thaliana plants exposed to Cd. | Notes: | E-mail: ann.cuypers@uhasselt.be | Keywords: | alternative oxidase; alternative respiration; Arabidopsis thaliana; cadmium (Cd); ethylene; oxidative challenge | Document URI: | http://hdl.handle.net/1942/18494 | ISSN: | 0022-0957 | e-ISSN: | 1460-2431 | DOI: | 10.1093/jxb/erv035 | ISI #: | 000356223900014 | Rights: | © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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J. Exp. Bot.-2015-Keunen-2967-77.pdf | Published version | 1.79 MB | Adobe PDF | View/Open |
JXB_Keunen et al_AOX1a_revised.pdf | Peer-reviewed author version | 1.94 MB | Adobe PDF | View/Open |
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