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Title: | Melittin disruption of raft and non-raft-forming biomimetic membranes: A study by quartz crystal microbalance with dissipation monitoring | Authors: | LOSADA-PEREZ, Patricia KHORSHID, Mehran HERMANS, Chris Robijns, T. PEETERS, Marloes Jimenez-Monroy, K. L. Truong, L. T. N. WAGNER, Patrick |
Issue Date: | 2014 | Publisher: | ELSEVIER SCIENCE BV | Source: | COLLOIDS AND SURFACES B-BIOINTERFACES, 123, p. 938-944 | Abstract: | In this work we examine the role of lateral phase separation in cholesterol-containing biomimetic membranes on the disrupting action of melittin using a label-free surface-sensitive technique, quartz crystal microbalance with dissipation monitoring (QCM-D). Melittin disruption mechanisms depend strongly on the geometry of the lipid layer; however, despite the interplay between layer geometry/thickness and melittin activity, results indicate that the presence of lipid heterogeneity and lateral phase separation greatly influences the disrupting efficiency of melittin. In homogeneous non-raft forming membranes with high cholesterol content, melittin spontaneous activity is strongly delayed compared to heterogeneous raft-forming systems with the same amount of cholesterol. These results confirm the importance of lateral phase separation as a determinant factor in peptide activity. The information provided can be used for the design of more efficient antimicrobial peptides and the possibility of using a label-free approach for tailored-membranes and interactions with other types of peptides, such as amyloid peptides. (C) 2014 Elsevier B.V. All rights reserved. | Notes: | [Losada-Perez, P.; Khorshid, M.; Hermans, C.; Robijns, T.; Peeters, M.; Jimenez-Monroy, K. L.; Wagner, P.] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Losada-Perez, P.; Wagner, P.] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Truong, L. T. N.] Hanoi Univ Sci & Technol 1, Sch Engn Phys, Hanoi, Vietnam. | Keywords: | Melittin; Lipid rafts; Cholesterol; Quartz crystal microbalance with dissipation;melittin; lipid rafts; cholesterol; quartz crystal microbalance with dissipation | Document URI: | http://hdl.handle.net/1942/18669 | ISSN: | 0927-7765 | e-ISSN: | 1873-4367 | DOI: | 10.1016/j.colsurfb.2014.10.048 | ISI #: | 000347580500118 | Rights: | © 2014 Elsevier B.V. All rights reserved. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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