Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/18993| Title: | An effective plasma membrane proteomics approach for small tissue samples | Authors: | Smolders, Katrien Lombaert, Nathalie VALKENBORG, Dirk Baggerman, Geert Arckens, Lutgarde |
Issue Date: | 2015 | Publisher: | NATURE PUBLISHING GROUP | Source: | SCIENTIFIC REPORTS, 5 | Abstract: | Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue. | Notes: | [Smolders, Katrien; Lombaert, Nathalie; Arckens, Lutgarde] Katholieke Univ Leuven, Dept Biol, Lab Neuroplast & Neuroprote, Leuven, Belgium. [Valkenborg, Dirk; Baggerman, Geert] Vlaamse Instelling Technol Onderzoek, Unit Environm Risk & Hlth, B-2400 Mol, Belgium. [Valkenborg, Dirk; Baggerman, Geert] UAntwerp, Ctr Prote, Antwerp, Belgium. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. | Document URI: | http://hdl.handle.net/1942/18993 | ISSN: | 2045-2322 | e-ISSN: | 2045-2322 | DOI: | 10.1038/srep10917 | ISI #: | 000355865400001 | Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
| Appears in Collections: | Research publications |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| smolders 1.pdf | Published version | 461.83 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
17
checked on Sep 5, 2020
WEB OF SCIENCETM
Citations
25
checked on Apr 30, 2024
Page view(s)
158
checked on Sep 7, 2022
Download(s)
172
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.