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Title: | Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5 | Authors: | SAENEN, Eline HOREMANS, Nele VANHOUDT, Nathalie VANDENHOVE, Hildegarde BIERMANS, Geert Van Hees, May Wannijn, Jean VANGRONSVELD, Jaco CUYPERS, Ann |
Issue Date: | 2015 | Publisher: | MDPI AG | Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16 (6), p. 12405-12423 | Abstract: | To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U at pH 7.5. Arabidopsis thaliana plants (Mouse-ear Cress) were exposed for three days to different U concentrations at pH 7.5. In the roots, the increased capacities of ascorbate peroxidase and glutathione reductase indicate an important role for the ascorbate-glutathione cycle during U-induced stress. However, a significant decrease in the ascorbate redox state was observed after exposure to 75 and 100 mu M U, indicating that those roots are severely stressed. In accordance with the roots, the ascorbate-glutathione cycle plays an important role in the antioxidative defence systems in A. thaliana leaves exposed to U at pH 7.5 as the ascorbate and glutathione biosynthesis were upregulated. In addition, small inductions of enzymes of the antioxidative defence system were observed at lower U concentrations to counteract the U-induced stress. However, at higher U concentrations it seems that the antioxidative defence system of the leaves collapses as reductions in enzyme activities and gene expression levels were observed. | Notes: | [Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean] Belgian Nucl Res Ctr SCK CEN, Biosphere Impact Studies, B-2400 Mol, Belgium. [Saenen, Eline; Biermans, Geert; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. | Keywords: | uranium toxicity; Arabidopsis thaliana; oxidative stress; gene expression; ascorbate-glutathione cycle | Document URI: | http://hdl.handle.net/1942/19159 | ISSN: | 1661-6596 | e-ISSN: | 1422-0067 | DOI: | 10.3390/ijms160612405 | ISI #: | 000357492800035 | Rights: | © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2016 |
Appears in Collections: | Research publications |
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